Boos J
Department of Pediatrics, University of Münster, FRG.
J Pharm Biomed Anal. 1991;9(1):47-52. doi: 10.1016/0731-7085(91)80236-3.
A sensitive isocratic ion-pair HPLC method using a reversed-phase C18 column and phosphate buffer at pH 6 for the determination of the cytotoxic intracellular anabolite 1-beta-D-arabinofuranosylcytosine-triphosphate (Ara-CTP) of the antineoplastic drug cytarabine in leukaemic cells in vivo is described. Simultaneous determination of the physiological nucleotide deoxycytidine-triphosphate is possible. The recovery from cells is greater than 90%, the limit of detection is 25 ng ml-1 (51 nM l-1) and about 10 pM mg-1 cell protein. Extraction procedure and pitfalls are discussed. The method enables intracellular drug monitoring of Ara-CTP with standard HPLC equipment.
描述了一种灵敏的等度离子对高效液相色谱法，该方法使用反相C18柱和pH 6的磷酸盐缓冲液，用于测定体内白血病细胞中抗肿瘤药物阿糖胞苷的细胞毒性细胞内代谢产物1-β-D-阿拉伯呋喃糖基胞嘧啶三磷酸（Ara-CTP）。同时测定生理核苷酸脱氧胞苷三磷酸是可行的。细胞回收率大于90%，检测限为25 ng ml-1（51 nM l-1）和约10 pM mg-1细胞蛋白。讨论了提取程序和注意事项。该方法可使用标准高效液相色谱设备对Ara-CTP进行细胞内药物监测。
