Roselli C E, West N B, Brenner R M
Department of Physiology, Oregon Health Sciences University, Portland 97201.
Biol Reprod. 1991 Apr;44(4):739-45. doi: 10.1095/biolreprod44.4.739.
We measured androgen receptors (AR) and 5 alpha-reductase activity (5 alpha RA) in the ductuli efferentes and epididymides from adult rhesus macaques. Tissue samples were either assayed biochemically for AR or stained immunocytochemically (ICC) with a monoclonal antibody against AR. To estimate 5 alpha RA, tissue microsomes were incubated with [1 alpha,2 alpha-3H]testosterone, and the [3H]dihydrotestosterone formed was quantified. We found significant regional differences in the levels of both 5 alpha RA and AR in the excurrent ducts. In general, both enzyme activity and AR levels were higher in the caput and corpus epididymis than in ductuli efferentes and cauda epididymis. With ICC, positive nuclear AR staining was detected in all epithelial cell types, whereas variable numbers of stromal cells were positively stained. Our data demonstrate that there are segmental differences in the concentrations of 5 alpha RA and AR in epididymis and suggest that there may be regional differences in the regulation of epididymal functions by androgen.
我们检测了成年恒河猴输出小管和附睾中的雄激素受体（AR）及5α-还原酶活性（5α RA）。组织样本要么进行AR的生化检测，要么用抗AR单克隆抗体进行免疫细胞化学（ICC）染色。为了评估5α RA，将组织微粒体与[1α,2α-3H]睾酮一起孵育，然后对生成的[3H]双氢睾酮进行定量。我们发现，在输出管道中，5α RA和AR水平存在显著的区域差异。一般来说，附睾头和附睾体中的酶活性及AR水平均高于输出小管和附睾尾。通过ICC检测，在所有上皮细胞类型中均检测到细胞核AR阳性染色，而基质细胞的阳性染色数量各不相同。我们的数据表明，附睾中5α RA和AR的浓度存在节段性差异，并提示雄激素对附睾功能的调节可能存在区域差异。
