West N B, Brenner R M
Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
Biol Reprod. 1990 Mar;42(3):533-8. doi: 10.1095/biolreprod42.3.533.
We obtained the testes, ductuli efferentes, and epididymides from adult rhesus and cynomolgus macaques and examined these tissues for estrogen receptors (ER) with immunocytochemistry (ICC) and a sucrose gradient assay. Both techniques employed monoclonal antibodies prepared against ER, and both showed that high concentrations of ER were present OFFy in the ductuli efferentes. Moreover, all specific staining was confined to the nuclei of the nonciliated, absorptive epithelial cells. The quantity of salt-extractable ER in the ductuli efferentes (834 +/- 161 [SEM] fmol/mg DNA [n = 8]) did not differ significantly from the amounts measured with the identical assay in oviducts and endometrium of estrogenized female macaques. Testes and epididymides of macaques had no specific staining by ICC and barely detectable amounts by biochemical analysis (7 +/- 4 [n = 3], 8 +/- 2 [n = 5], 33 +/- 16 [n = 3], and 6 +/- 3 [n = 8] fmol/mg DNA for testis and caput, corpus, and cauda epididymis, respectively). The functional significance of the high levels of ER in the ductuli efferentes of macaques remains to be determined.
我们从成年恒河猴和食蟹猕猴身上获取了睾丸、输出小管和附睾，并用免疫细胞化学（ICC）和蔗糖梯度分析法对这些组织进行雌激素受体（ER）检测。这两种技术均采用针对ER制备的单克隆抗体，且均显示输出小管中存在高浓度的ER。此外，所有特异性染色均局限于非纤毛吸收性上皮细胞的细胞核。输出小管中盐可提取的ER量（834±161[标准误]fmol/mg DNA[n = 8]）与用相同检测方法在雌激素化雌性猕猴的输卵管和子宫内膜中测得的量无显著差异。猕猴的睾丸和附睾经ICC检测无特异性染色，生化分析检测到的量极少（睾丸、附睾头、附睾体和附睾尾分别为7±4[n = 3]、8±2[n = 5]、33±16[n = 3]和6±3[n = 8]fmol/mg DNA）。猕猴输出小管中高水平ER的功能意义尚待确定。
