Arenas M I, Royuela M, Lobo M V, Alfaro J M, Fraile B, Paniagua R
Department of Cell Biology and Genetics, University of Alcalá, Madrid, Spain.
J Anat. 2001 Oct;199(Pt 4):465-72. doi: 10.1046/j.1469-7580.2001.19940465.x.
Expression of androgen receptor (AR), estrogen receptor alpha (ER-alpha) and estrogen receptor beta (ER-beta) in the testis of the marbled newt (Triturus marmoratus marmoratus) was investigated, with special attention to changes during the annual testicular cycle, using light microscopy immunohistochemistry and Western blot analysis. Primordial germ cells, primary and secondary spermatogonia and spermatocytes showed a positive reaction to the 3 receptor antibodies during the annual reproductive cycle. Follicular cells were positive to AR, ER-alpha and ER-beta during the spermiogenesis and quiescence periods in the glandular tissue. Interstitial cells showed reactivity to AR, ER-alpha and ER-beta in the spermiogenesis and the quiescence periods, and presented no labelling to these receptors in the proliferative period. These findings suggest that, as in mammals, there is an androgen-estrogen regulation of the function and development of the newt testis.
利用光学显微镜免疫组织化学和蛋白质印迹分析，研究了大理石纹螈（Triturus marmoratus marmoratus）睾丸中雄激素受体（AR）、雌激素受体α（ER-α）和雌激素受体β（ER-β）的表达情况，特别关注年度睾丸周期中的变化。在年度生殖周期中，原始生殖细胞、初级和次级精原细胞以及精母细胞对这三种受体抗体均呈阳性反应。在腺组织的精子发生期和静止期，滤泡细胞对AR、ER-α和ER-β呈阳性。间质细胞在精子发生期和静止期对AR、ER-α和ER-β有反应，而在增殖期对这些受体无标记。这些发现表明，与哺乳动物一样，蝾螈睾丸的功能和发育存在雄激素 - 雌激素调节。
