Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Am J Trop Med Hyg. 2010 May;82(5):889-98. doi: 10.4269/ajtmh.2010.09-0711.
Rats are known to be the most important reservoirs and transmission sources of leptospirosis. However, the status of leptospirosis in the Philippines regarding reservoirs and transmission remains unknown. A survey was conducted in Metro Manila and Laguna that analyzed samples obtained from 106 rats. Using the microscopic agglutination test, we found that 92% of rat serum samples were positive for anti-Leptospira antibodies; the most common infecting serovars were Manilae, Hebdomadis, and Losbanos. On the basis of pulsed-field gel electrophoresis and gyrase B gene sequence analyses, four groups of rat kidney isolates were found: L. interrogans serovar Manilae, serovar Losbanos, and serogroup Grippotyphosa, and L. borgpetersenii serogroup Javanica. Most isolates were lethal after experimental infection of golden Syrian hamsters. Results showed that these four Leptospira serovars and serogroups are circulating among rats, and that these animals may be one of the possible transmission sources of leptospirosis in the Philippines.
已知老鼠是钩端螺旋体病最重要的储存宿主和传播源。然而,菲律宾关于钩端螺旋体病的储存宿主和传播源的情况尚不清楚。在马尼拉和拉古纳进行了一项调查,分析了从 106 只老鼠中获得的样本。使用显微镜凝集试验,我们发现 92%的鼠血清样本对钩端螺旋体抗体呈阳性;最常见的感染血清型为马尼拉型、半月型和洛斯巴诺斯型。根据脉冲场凝胶电泳和拓扑异构酶 B 基因序列分析,发现 4 组鼠肾分离株:问号钩端螺旋体血清型马尼拉型、血清型洛斯巴诺斯型和血清群 grippotyphosa 型,以及伯氏包柔螺旋体血清群爪哇型。大多数分离株在实验感染金黄地鼠后具有致死性。结果表明,这四种钩端螺旋体血清型和血清群在老鼠中流行,这些动物可能是菲律宾钩端螺旋体病的潜在传播源之一。