Wang Shuyue, Yu Yongxin, Cui Mingxiao, Liu Kehai, Liu Kewu
Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
National Experimental Teaching Demonstration Canter for Food Science and Engineering, Shanghai Ocean University, Shanghai, China.
Front Nutr. 2021 Dec 24;8:796175. doi: 10.3389/fnut.2021.796175. eCollection 2021.
Natural edible oil derived from wild non-cultivated oil crops contributed to human daily nutritional diversity and disease prevention. It was important to investigate the nutritional value of these oils and the feasibility of crop cultivation. The present study focused on the assessment of seed oil quality of Hance (SWH) and its molecular breeding. Wild SWH seed oil was extracted by supercritical CO technology and the composition of the oil was determined by using gas chromatography mass spectrometry (GC-MS) analysis. The oil content of SWH seeds reaches around 40%. Its seed oil was found to be rich in unsaturated fatty acids, such as 24.24% of linolenic acid and 50.56% of linoleic acid, and vitamin E (25.92 mg kg). The cytotoxicity and heavy metal analysis showed SWH seed oil was safe for consumption. In addition, the SWH strains with excellent characteristics were screened out for cultivation according to genetic diversity and morphological analysis. Amplified fragment length polymorphism (AFLP) markers were used to evaluate the genetic diversity of 28 accessions of wild SWH seeds and 5 accessions were selected to cultivate. Among them, two strains of SWH (sample 3 and 6) with high yielding (275.7 and 266.8 area yield kg) were suitable for dense planting and could be used to establish the raw material forest of SWH seed oil. The results of this study indicated the potential of development of selected SWH as novel oil crops and their wide cultivation.
源自野生非栽培油料作物的天然食用油有助于人类日常营养多样性和疾病预防。研究这些油的营养价值以及作物种植的可行性很重要。本研究聚焦于华南锥(SWH)种子油品质评估及其分子育种。采用超临界CO₂技术提取野生华南锥种子油,并通过气相色谱 - 质谱联用(GC - MS)分析确定油的成分。华南锥种子的含油量约为40%。发现其种子油富含不饱和脂肪酸,如24.24%的亚麻酸和50.56%的亚油酸,以及维生素E(25.92毫克/千克)。细胞毒性和重金属分析表明华南锥种子油食用安全。此外,根据遗传多样性和形态分析筛选出具有优良特性的华南锥菌株用于种植。利用扩增片段长度多态性(AFLP)标记评估了28份野生华南锥种子的遗传多样性,并选择了5份进行种植。其中,两个高产的华南锥菌株(样品3和6,单位面积产量分别为275.7和266.8千克)适合密植,可用于建立华南锥种子油原料林。本研究结果表明所选华南锥作为新型油料作物开发及其广泛种植的潜力。