Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, School of Materials Science and Engineering, Shandong University, Jinan 250061, People's Republic of China.
ACS Appl Mater Interfaces. 2011 Nov;3(11):4354-62. doi: 10.1021/am201008n. Epub 2011 Nov 3.
A novel amperometric biosensor based on the BNNTs-Pani-Pt hybrids with Pt nanoparticle homogeneously decorated on polyaniline (Pani)-wrapped boron nitride nanotubes (BNNTs), was developed. It is shown that π interactions take place between BNNTs and polyaniline (Pani) located at N atoms from BNNTs and C atoms from Pani, resulting in the water solubility for the Pani wrapped BNNTs hybrids. The developed glucose biosensor displayed high sensitivity and stability, good reproducibility, anti-interference ability, especially excellent acid stability and heat resistance. The resulted BNNTs-Pani-Pt hybrid amperometric glucose biosensor exhibited a fast response time (within 3 s) and a linear calibration range from 0.01 to 5.5 mM with a high sensitivity and low detection limit of 19.02 mA M(-1) cm(-2) and 0.18 μM glucose (S/N = 3). Surprisedly, the relative activity of the GC/BNNTs-Pani-Pt-GOD electrode keeps almost no change in a range from pH 3 to 7. Futhermore, the BNNTs-Pani-Pt hybrid biosensor maintains a high GOD enzymatic activity even at a relatively high temperature of 60 °C. This might be attributed to the effect of electrostatic field and hydrophobia of BNNTs. The unique acid stability and heat resistance of this sensor indicate great promising application in numerous industrial and biotechnological operations involving harsh conditions.
一种新型的基于 BNNTs-Pani-Pt 杂化材料的安培生物传感器被开发出来，其中 Pt 纳米粒子均匀地分布在聚吡咯（Pani）包裹的氮化硼纳米管（BNNTs）上。研究表明，BNNTs 和聚吡咯（Pani）之间存在π相互作用，这种作用发生在 BNNTs 上的 N 原子和 Pani 上的 C 原子之间，从而使 Pani 包裹的 BNNTs 杂化材料具有水溶性。所开发的葡萄糖生物传感器具有高灵敏度和稳定性、良好的重现性、抗干扰能力，特别是具有优异的耐酸性和耐热性。所得的 BNNTs-Pani-Pt 杂化电流型葡萄糖生物传感器具有快速的响应时间（在 3 s 内）和从 0.01 到 5.5 mM 的线性校准范围，灵敏度和检测限分别为 19.02 mA M(-1) cm(-2)和 0.18 μM 葡萄糖（S/N = 3）。令人惊讶的是，GC/BNNTs-Pani-Pt-GOD 电极的相对活性在 pH 3 到 7 的范围内几乎没有变化。此外，即使在相对较高的温度 60°C 下，BNNTs-Pani-Pt 杂化生物传感器也能保持较高的 GOD 酶活性。这可能归因于 BNNTs 的静电场和疏水性的影响。该传感器独特的耐酸性和耐热性表明，它在涉及苛刻条件的许多工业和生物技术操作中有很大的应用前景。
