Spine Unit, Royal Adelaide Hospital, The University of Adelaide, Adelaide, South Australia.
Spine (Phila Pa 1976). 2010 May 20;35(12):1200-5. doi: 10.1097/BRS.0b013e3181c02812.
A prospective immunohistological study in an animal model.
To identify and describe the phenotype of neoinnervation in experimental anular tears.
Controversy surrounds neoinnervation of degenerate discs which has been proposed as the anatomic basis for discogenic pain. Ablation of neoinnervation has been postulated as the theoretical basis for the claimed successes of procedures such as intradiscal electrotherapy. The animal model of disc degeneration previously developed in our research center provides an opportunity to investigate the innervation of anular tears in an extensively characterized lesion.
A surgical anular tear was created in 5 lumbar discs in 11 sheep which were killed at 1, 2, 3, and 12 months. Each spine was x-rayed and divided into motion segments for histologic analysis. Serial sections through the tear were immunostained for protein gene product 9.5, tyrosine hydroxylase, and calcitonin gene receptor protein.
Neoinnervation of the periphery of the anular tear was observed. Ingrowing nerves penetrated marginally deeper than the normal anular innervation but no nerves were identified in the inner anulus or nucleus. A minority of the new axons were calcitonin gene receptor protein or tyrosine hydroxylase positive.
The anulus tears in this model are innervated only peripherally to a depth only marginally greater than that of the normal anulus.
动物模型中的前瞻性免疫组织学研究。
鉴定和描述实验性环状撕裂中新的神经支配的表型。
退化椎间盘的新生神经支配存在争议，有人提出这是椎间盘源性疼痛的解剖学基础。新生神经的消融被认为是椎间盘内电疗等已被声称成功的手术的理论基础。我们研究中心之前开发的椎间盘退变动物模型为广泛特征化病变中环状撕裂的神经支配提供了研究机会。
在 11 只绵羊的 5 个腰椎间盘上创建了一个手术性环状撕裂，然后在 1、2、3 和 12 个月时将其杀死。对每个脊柱进行 X 射线检查，并将其分为用于组织学分析的运动节段。通过免疫组织化学染色对蛋白基因产物 9.5、酪氨酸羟化酶和降钙素基因受体蛋白对撕裂的连续切片进行染色。
观察到环状撕裂周边有新生神经支配。侵入的神经仅略微深入正常环状神经支配的边缘，但在内部环状或核中未发现神经。少数新轴突对降钙素基因受体蛋白或酪氨酸羟化酶呈阳性。
在这个模型中，环状撕裂仅在周边有神经支配，深度仅比正常环状稍深。
