Schäfer E, Dummer R, Eilles C, Börner W, Martin R, Rendl J, Burg G
Department of Nuclear Medicine, University of Würzburg, Federal Republic of Germany.
Eur J Nucl Med. 1991;18(2):106-10. doi: 10.1007/BF00950755.
In patients with metastatic malignant melanoma the distribution patterns of radiolabelled lymphokine-activated killer (LAK) cells were investigated. Peripheral mononuclear cells (PMC) were isolated from six patients. LAK cells were generated by culturing PMC in complete medium containing 1000 U interleukin (IL)-2/ml and labelled with indium 111 before retransfer. We obtained scans at 2.5, 24, 48 or 96 h after injection with a high resolution gamma-camera. Intravenously injected LAK cells distributed to the lungs, liver, spleen and bone marrow. External tumour detection of known lymph node and bone metastases was successful in four. It failed in one patient with a solitary lung metastasis and in another patient with subcutaneous metastases. Our results suggest that LAK cells show tumour homing, providing a direct interaction between tumour and cytotoxic cells. We conclude that PMC seem to retain their ability to migrate after IL-2 stimulation and 111In-labeling. This technique may be helpful for kinetics studies or external detection of metastases in patients with malignant melanoma.
对转移性恶性黑色素瘤患者放射性标记的淋巴因子激活的杀伤（LAK）细胞的分布模式进行了研究。从6例患者中分离出外周血单个核细胞（PMC）。通过在含1000 U白细胞介素（IL）-2/ml的完全培养基中培养PMC来生成LAK细胞，并在回输前用铟111进行标记。在注射后2.5、24、48或96小时用高分辨率γ相机进行扫描。静脉注射的LAK细胞分布到肺、肝、脾和骨髓。4例成功检测到已知的淋巴结和骨转移的外部肿瘤。1例孤立性肺转移患者和另1例皮下转移患者检测失败。我们的结果表明，LAK细胞显示出肿瘤归巢，在肿瘤与细胞毒性细胞之间提供了直接相互作用。我们得出结论，PMC在IL-2刺激和铟111标记后似乎保留了其迁移能力。该技术可能有助于恶性黑色素瘤患者转移灶的动力学研究或外部检测。
