日本实验兔中梨形芽孢杆菌感染的血清学调查。

A serological survey on Bacillus piliformis infection in laboratory rabbits in Japan.

作者信息

Goto K, Itoh T, Takakura A, Kunita S, Terada E, Kagiyama N

机构信息

Central Institute for Experimental Animals, Kanagawa, Japan.

出版信息

Jikken Dobutsu. 1991 Apr;40(2):231-3. doi: 10.1538/expanim1978.40.2_231.

DOI:10.1538/expanim1978.40.2_231

PMID:2044670

Abstract

A total of 544 rabbit sera obtained from 6 commercial breeding facilities and 9 research institutions during 1985-1990 were tested for Bacillus piliformis antibody by an enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent antibody test (IFAT). The antibody was detected in 53 (14.2%) rabbits from 3 breeding facilities and 30 (17.4%) rabbits from 6 research institutions, indicating the prevalence of B. piliformis infection among laboratory rabbits in Japan. The overall agreement with ELISA for immune status was 96.9% (527/544) with IFAT. In tests of the ability of ELISA and IFAT to quantitate antibody, a correlation coefficient (r) of 0.86 (P less than 0.01) was obtained by plotting the measured average log of the ELISA titer against the corresponding log of the IFAT titer.

摘要

1985年至1990年期间，从6个商业养殖设施和9个研究机构收集了544份兔血清，采用酶联免疫吸附测定（ELISA）和间接免疫荧光抗体试验（IFAT）检测兔梨形芽孢杆菌抗体。在来自3个养殖设施的53只（14.2%）兔子和来自6个研究机构的30只（17.4%）兔子中检测到了抗体，这表明日本实验兔中存在兔梨形芽孢杆菌感染。ELISA与IFAT在免疫状态检测方面的总体一致性为96.9%（527/544）。在ELISA和IFAT定量抗体能力的测试中，通过绘制ELISA滴度的测量平均对数与IFAT滴度的相应对数，得到相关系数（r）为0.86（P<0.01）。