日本实验兔中梨形芽孢杆菌感染的血清学调查。
A serological survey on Bacillus piliformis infection in laboratory rabbits in Japan.
作者信息
Goto K, Itoh T, Takakura A, Kunita S, Terada E, Kagiyama N
机构信息
Central Institute for Experimental Animals, Kanagawa, Japan.
出版信息
Jikken Dobutsu. 1991 Apr;40(2):231-3. doi: 10.1538/expanim1978.40.2_231.
A total of 544 rabbit sera obtained from 6 commercial breeding facilities and 9 research institutions during 1985-1990 were tested for Bacillus piliformis antibody by an enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent antibody test (IFAT). The antibody was detected in 53 (14.2%) rabbits from 3 breeding facilities and 30 (17.4%) rabbits from 6 research institutions, indicating the prevalence of B. piliformis infection among laboratory rabbits in Japan. The overall agreement with ELISA for immune status was 96.9% (527/544) with IFAT. In tests of the ability of ELISA and IFAT to quantitate antibody, a correlation coefficient (r) of 0.86 (P less than 0.01) was obtained by plotting the measured average log of the ELISA titer against the corresponding log of the IFAT titer.
1985年至1990年期间，从6个商业养殖设施和9个研究机构收集了544份兔血清，采用酶联免疫吸附测定（ELISA）和间接免疫荧光抗体试验（IFAT）检测兔梨形芽孢杆菌抗体。在来自3个养殖设施的53只（14.2%）兔子和来自6个研究机构的30只（17.4%）兔子中检测到了抗体，这表明日本实验兔中存在兔梨形芽孢杆菌感染。ELISA与IFAT在免疫状态检测方面的总体一致性为96.9%（527/544）。在ELISA和IFAT定量抗体能力的测试中，通过绘制ELISA滴度的测量平均对数与IFAT滴度的相应对数，得到相关系数（r）为0.86（P<0.01）。
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