Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
J Clin Periodontol. 2010 Apr;37(4):374-81. doi: 10.1111/j.1600-051X.2010.01539.x.
The aim of the present study was to evaluate the effect of a basic fibroblast growth factor (bFGF) candidate treatment on periodontal healing in two-wall intrabony defects in dogs.
Two-wall intrabony defects (5 x 5 x 5 mm) were created surgically on the distal and mesial sides of bilateral mandibular second and fourth premolars in four Beagle dogs. bFGF, enamel matrix derivative (EMD) and platelet-derived growth factor with beta-tricalcium phosphate (PDGF/beta-TCP) treatments, and sham-surgery (OFD) were rotated among the four defects in each animal, EMD and PDGF/beta-TCP serving as benchmark controls. The animals were euthanized for radiographic and histologic evaluation at 8 weeks.
Bone formation was significantly greater in the bFGF group (4.11 +/- 0.77 mm) than in the EMD (3.32 +/- 0.71 mm; p<0.05) and OFD (3.09 +/- 0.52 mm; p<0.01) groups. The EMD (4.59 +/- 1.19 mm) and PDGF/beta-TCP (4.66 +/- 0.7 mm) groups exhibited significantly greater cementum regeneration with periodontal ligament-like tissue than the OFD group (2.96 +/- 0.69 mm; p<0.01). No significant differences were observed between the bFGF and the PDGF/beta-TCP groups in any of the histometric parameters.
The candidate bFGF treatment supported periodontal regeneration comparable with that of established benchmarks: EMD and PDGF/beta-TCP.
本研究旨在评估碱性成纤维细胞生长因子（bFGF）候选物治疗对犬双壁骨内缺损牙周愈合的影响。
在 4 只比格犬双侧下颌第二和第四前磨牙的远中和近中侧，通过手术制备双壁骨内缺损（5 x 5 x 5 mm）。bFGF、釉基质衍生物（EMD）和血小板衍生生长因子与β-磷酸三钙（PDGF/β-TCP）治疗以及假手术（OFD）在每只动物的四个缺损之间轮换，EMD 和 PDGF/β-TCP 作为基准对照。在 8 周时，对动物进行放射学和组织学评估以处死。
bFGF 组（4.11 +/- 0.77 mm）的骨形成明显大于 EMD 组（3.32 +/- 0.71 mm；p<0.05）和 OFD 组（3.09 +/- 0.52 mm；p<0.01）。EMD 组（4.59 +/- 1.19 mm）和 PDGF/β-TCP 组（4.66 +/- 0.7 mm）与 OFD 组（2.96 +/- 0.69 mm；p<0.01）相比，具有牙周韧带样组织的牙骨质再生明显更大。在任何组织学参数中，bFGF 组和 PDGF/β-TCP 组之间均未观察到显著差异。
候选 bFGF 治疗支持与已建立的基准（EMD 和 PDGF/β-TCP）相当的牙周再生。
