Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva, Israel.
Fertil Steril. 2011 Mar 15;95(4):1267-71. doi: 10.1016/j.fertnstert.2010.03.051. Epub 2010 May 5.
To investigate the in vitro effect of neurotrophin 4 (NT-4) on follicular assembly in human fetal ovaries.
Human ovarian tissue from fetuses at 19-20 gestational weeks was placed in organ culture for two weeks with NT-4. Control groups were cultured with a neutralizing antibody against NT-4.
Infertility unit at an university-affiliated tertiary medical center.
PATIENT(S): Four patients who underwent pregnancy terminations at 19-20 gestational weeks.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Histologic findings of follicular assembly.
RESULT(S): Follicular assembly was significantly increased in the specimens cultured with NT-4 than in the uncultured specimens, the samples cultured without NT-4, and samples cultured with the neutralizing antibody. In the second week of culture, additional follicular assembly was promoted in the presence of 100 ng/mL NT-4 but not with 10 ng/mL NT-4.
CONCLUSION(S): This is the first report showing that NT-4 seems to promote human follicular assembly in fetal ovaries, probably in a dose-dependent manner. Follicular assembly is regulated by multiple signals, and additional studies on the effects of other growth factors in combination with NT-4 are warranted.
研究神经营养因子 4（NT-4）对人胎卵巢滤泡组装的体外作用。
将妊娠 19-20 周的胎儿卵巢组织置于器官培养物中培养两周，并加入 NT-4。对照组则用 NT-4 的中和抗体进行培养。
大学附属三级医疗中心的不孕不育科。
4 名在妊娠 19-20 周时终止妊娠的患者。
无。
滤泡组装的组织学发现。
与未培养的标本、未加 NT-4 培养的标本和加中和抗体培养的标本相比，加 NT-4 培养的标本中滤泡组装明显增加。在培养的第 2 周，100ng/ml 的 NT-4 促进了额外的滤泡组装，但 10ng/ml 的 NT-4 则没有这种作用。
这是首个表明 NT-4 似乎以剂量依赖的方式促进人胎卵巢滤泡组装的报告。滤泡组装受多种信号调节，因此有必要进一步研究其他生长因子与 NT-4 联合作用的影响。
