Waraksa J A, Lindsay R M, Ip N Y, Hutz R J
University of Wisconsin-Milwaukee, Department of Biological Sciences, 53201-0413, USA.
Zoolog Sci. 1995 Aug;12(4):499-502. doi: 10.2108/zsj.12.499.
Innervation of the ovary is known to be affected by neurotrophic factors; mRNA for some of these molecules and their low-affinity receptors have been identified in ovarian follicular tissue. We attempted to determine whether neurotrophin (NT-3) exerts a non-neuronal impact on the ovary, affecting follicular estradiol secretion via a possible humoral action. Ovaries were excised from mature Golden Syrian Hamsters (Mesocricetus auratus) and large preovulatory follicles were removed by microdissection. Follicles were incubated for 72 hr in control medium, or medium containing 10 ng, 100 ng, or 1000 ng/ml NT-3. After culture, medium samples were assayed for estradiol content. NT-3 exerted dichotomous effects, depending on concentration: NT-3 augmented mean estradiol output two-fold at 100 ng/ml, while 1000 ng/ml NT-3 decreased estradiol secretion back to control levels. By 48-72 hr in culture, all groups exhibited a significant decline in mean estradiol secretion due presumably to diminished viability, independent of neurotrophin action. These data suggest a humoral role for NT-3 in estradiol secretion of preovulatory follicles in culture.
已知卵巢的神经支配会受到神经营养因子的影响；在卵巢滤泡组织中已鉴定出其中一些分子及其低亲和力受体的mRNA。我们试图确定神经营养蛋白（NT-3）是否对卵巢产生非神经元影响，通过可能的体液作用影响滤泡雌二醇分泌。从成熟的金黄地鼠（Mesocricetus auratus）中切除卵巢，并通过显微切割去除大型排卵前滤泡。将滤泡在对照培养基或含有10 ng、100 ng或1000 ng/ml NT-3的培养基中孵育72小时。培养后，测定培养基样品中的雌二醇含量。NT-3根据浓度产生二分效应：NT-3在100 ng/ml时使平均雌二醇产量增加两倍，而1000 ng/ml NT-3使雌二醇分泌降至对照水平。在培养48 - 72小时后，所有组的平均雌二醇分泌均显著下降，推测这是由于活力下降所致，与神经营养因子的作用无关。这些数据表明NT-3在培养的排卵前滤泡的雌二醇分泌中具有体液作用。
