Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, India.
Colloids Surf B Biointerfaces. 2010 Aug 1;79(1):164-73. doi: 10.1016/j.colsurfb.2010.03.049. Epub 2010 Apr 10.
The aim of this study was to investigate the haemocompatibility of poly(N-isopropylacrylamide)-co-poly(ethylene glycol), PNIPAAM-PEG based nanoparticles and the influence of poly(ethylene glycol), PEG on the interactions of nanoparticles with cells. To achieve this purpose, thermosensitive PNIPAAM-PEG nanoparticles were synthesized by free radical dispersion polymerization method. Optimized nanosystems had particle sizes less than 200 nm, low polydispersity and LCST of 40-41 degrees C. The nanoparticles also showed nearly 83% encapsulation efficiency for doxorubicin HCl with temperature dependent release. Presence of PEG resulted in reduced protein adsorption by more than 50% in comparison to non-PEG containing nanoparticles. Protein adsorption was noted to be dependent on PEG chain length and was the least with M(n)=4000. The particles up to a concentration of 2mg/ml did not show any toxicity on J774 and L929 cell lines. No interactions were observed when NIPAAM-PEG nanoparticles were incubated with blood cells viz. RBCs, neutrophils, platelets and the coagulation system suggesting their haemocompatibility.
本研究旨在探讨聚（N-异丙基丙烯酰胺）-共-聚（乙二醇）（PNIPAAM-PEG）基纳米粒子的血液相容性，以及聚乙二醇（PEG）对纳米粒子与细胞相互作用的影响。为了实现这一目的，采用自由基分散聚合方法合成了温敏性 PNIPAAM-PEG 纳米粒子。优化后的纳米系统粒径小于 200nm，多分散性低，LCST 为 40-41°C。纳米粒子对盐酸多柔比星的包封效率接近 83%，且具有温度依赖性释放。与不含 PEG 的纳米粒子相比，PEG 的存在使蛋白质吸附减少了 50%以上。蛋白质吸附与 PEG 链长有关，M(n)=4000 时最少。浓度高达 2mg/ml 的纳米粒子对 J774 和 L929 细胞系没有任何毒性。当 NIPAAM-PEG 纳米粒子与红细胞（RBCs）、嗜中性粒细胞、血小板和凝血系统等血液细胞孵育时，没有观察到相互作用，这表明它们具有血液相容性。
