DNA-多荧光团缔合物作为酯酶和脂肪酶的灵敏报告分子。
DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.
机构信息
Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
出版信息
Chem Commun (Camb). 2010 Feb 28;46(8):1221-3. doi: 10.1039/b926338a. Epub 2010 Jan 22.
Abstract
DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.
摘要
DNA 支架寡脱氧核苷酸荧光团（ODFs）被用作酶键断裂活性的开启感应的报告分子。通过酯键与 dabcyl 猝灭剂连接的芘脱氧核苷酸四聚体 ODF 具有很高的猝灭效率，并且在体外和完整的人细胞中与几种酶反应时产生了很大的信号增加。
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