Equipe de Chimie Bio-Organique, COBRA-CNRS UMR 6014 & FR 3038, rue Lucien Tesnière, 76131, Mont-Saint-Aignan, France.
Org Biomol Chem. 2010 Apr 21;8(8):1777-80. doi: 10.1039/b926316k.
This study focuses on the disassembly-behavior of self-immolative pro-fluorescent linkers under physiological conditions and through an enzyme-initiated domino reaction. The targeted linkers are based on para-aminobenzylalcohol (PABA) or hemithioaminal derivatives of para-carboxybenzaldehyde or glyoxilic acid. We found that a fine tuning of the kinetic properties could be obtained through the modulation of the linker structure, giving either a fast signal response or free-adaptable systems suitable for the design of protease-sensitive fluorogenic probes or prodrug systems.
本研究聚焦于在生理条件下通过酶引发的级联反应，对自耗荧光连接子的拆卸行为进行研究。目标连接子基于对氨基苯甲醇（PABA）或对羧基苯甲醛或乙醛酸的半硫代亚胺衍生物。我们发现，通过调节连接子结构，可以获得精细的动力学性能调整，从而得到快速的信号响应或自适应的系统，适用于蛋白酶敏感荧光探针或前药系统的设计。
