Cheng Litao, Guo Qiaosheng, Liu Zuoyi
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2010 Feb;35(4):405-10. doi: 10.4268/cjcmm20100401.
To study the arbuscular mycorrhiza and arbuscular mycorrhizal fungi associated with cultivated and wild Pinellia ternata in Guizhou province.
Wild and cultivated P. ternata roots were observed through staining and microscopic examination, the arbuscular mycorrhizal fungi spores were isolated through wet thieving according to Gerdemann & Nicolson (1963), the spores were identified following the description of Schenck & Pérez (1988), and some previous publications.
The typical arbuscular mycorrhiza (AM) structure was showed according to a research of wild and cultivated P. ternata. In the survey of AM fungi species in the rhizosphere of wild and cultivated P. ternata, 3 genera and 21 species were found, 3 genera and 7 species were identified. 5 species of them belong to Glomus, 1 species belongs to Scutellospora, 1 species belongs to Gigaspora, including Glomus mosseae, G. intraradices, G. melanosporum, G. deserticola, G. aggregatum, Scutellospora castanea, Gigaspora albida, and one of them was a new record, i.e., Scutellospora castanea which was the dominant species in Bijie.
The diversity of AM fungi between wild and cultivated Pinellia ternata was showed on this survey, the fungi associated with wild ones are different form the cultivated ones, such as Gigaspora albida only occurs in cultivated ones, Glomus melanosporum only occurs in wild ones, while Glomus mosseae and Glomus intraradices occur in both wild and cultivated ones, and there were specialization species in Bijie, all these can provide new though for solving degradation problem of cultivated Pinellia ternata.
研究贵州省栽培及野生半夏的丛枝菌根及丛枝菌根真菌。
通过染色和显微镜检查观察野生及栽培半夏的根系，依据Gerdemann和Nicolson（1963）的方法采用湿筛法分离丛枝菌根真菌孢子，参照Schenck和Pérez（1988）的描述及一些先前的文献对孢子进行鉴定。
对野生及栽培半夏的研究显示出典型的丛枝菌根（AM）结构。在对野生及栽培半夏根际AM真菌种类的调查中，发现3个属21个种，鉴定出3个属7个种。其中5个种属于球囊霉属，1个种属于盾巨孢囊霉属，1个种属于巨孢囊霉属，包括摩西球囊霉、根内球囊霉、黑孢球囊霉、沙漠球囊霉、聚生球囊霉、栗色盾巨孢囊霉、白色巨孢囊霉，其中有1个新记录种，即栗色盾巨孢囊霉，它是毕节的优势种。
本次调查显示了野生及栽培半夏中AM真菌的多样性，与野生半夏相关的真菌与栽培半夏不同，例如白色巨孢囊霉仅出现在栽培半夏中，黑孢球囊霉仅出现在野生半夏中，而摩西球囊霉和根内球囊霉在野生和栽培半夏中均有出现，且毕节存在特有种，所有这些可为解决栽培半夏的退化问题提供新思路。
