Cheng Yan, Ma Yao, Peng Ting, Wang Jue, Lin Ru, Cheng Hai-dong
Department of Obstetrics, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
Zhonghua Fu Chan Ke Za Zhi. 2010 Mar;45(3):170-3.
To elucidate the influence of fetal genotype in both non-diabetic gravidas and pregnant women on gestational diabetes mellitus (GDM) through analysis of the genotype discrepancy between maternal and fetal Pro12Ala single nucleotide polymorphism (SNP) of peroxisome proliferator-activated receptor gamma 2 (PPARG2) genes.
Pregnant women, who delivered in the Obstetrics and Gynecology Hospital of Fudan University from October 2005 to February 2007, and their newborn babies were selected, and were divided into GDM and control group. The GDM group consisted of 55 gravidas with GDM and 40 newborns born to the GDM mothers, and the control group consisted of 173 healthy gravidas and their 50 neonates. Polymerase chain reaction-denaturing high-performance liquid chromatography was applied to detect the distribution of PPARG2 Pro12Ala alleles in all subjects. The concentrations of plasma fasting blood sugar (FBS) and several bio-markers of lipids, including total cholesterol, triglyceride, apoprotein A, high-density lipoprotein and low-density lipoprotein, were also tested for the mothers.
(1) No significant difference was found in the frequencies of Pro/Pro genotype between the GDM mothers and control mothers (94.6% vs 90.8%, P > 0.05), nor between the GDM offspring and control offspring (95.0% vs 94.0%, P > 0.05) or between the GDM mothers and GDM offspring (P > 0.05). The same was shown in the frequencies of Pro/Ala genotype both between the GDM mothers and control mothers (5.5% vs 9.2%, P > 0.05) and between the GDM offspring and control offspring (2.5% vs 3.0%, P > 0.05). (2) Within both GDM and control group, the maternal FBS and various lipids concentrations of Pro/Pro genotype gravidas showed no significant difference compared to those of Pro/Ala genotype mothers (P > 0.05). (3) Based on the four possible PPARG2 genotype pairs between the mothers and fetuses, Pro/Pro mother and her Pro/Pro fetus, Pro/Ala mother and her Pro/Ala fetus, Pro/Ala mother and her Pro/Pro fetus, and Pro/Pro mother and her Pro/Ala fetus, less Pro/Pro pairs and more Pro/Ala pairs were found in the GDM group than in the control (72.5% vs 92.0%, P = 0.014; 27.5% vs 6.0%, P < 0.05).
Neither the maternal nor the offspring's Pro/Ala genotypes is associated with the genesis of GDM. However, the discrepancy of PPARG2 Pro12Ala polymorphism between mother and her fetus implies a possible cause of GDM.
通过分析过氧化物酶体增殖物激活受体γ2（PPARG2）基因的母亲和胎儿Pro12Ala单核苷酸多态性（SNP）之间的基因型差异，阐明胎儿基因型在非糖尿病孕妇和孕妇中对妊娠期糖尿病（GDM）的影响。
选取2005年10月至2007年2月在复旦大学附属妇产科医院分娩的孕妇及其新生儿，分为GDM组和对照组。GDM组由55例患有GDM的孕妇和40例GDM母亲所生的新生儿组成，对照组由173例健康孕妇及其50例新生儿组成。应用聚合酶链反应-变性高效液相色谱法检测所有受试者中PPARG2 Pro12Ala等位基因的分布。还对母亲进行了空腹血糖（FBS）浓度以及几种脂质生物标志物（包括总胆固醇、甘油三酯、载脂蛋白A、高密度脂蛋白和低密度脂蛋白）的检测。
（1）GDM母亲和对照母亲之间Pro/Pro基因型频率无显著差异（94.6%对90.8%，P>0.05），GDM后代和对照后代之间（95.0%对94.0%，P>0.05）或GDM母亲和GDM后代之间（P>0.05）也无显著差异。GDM母亲和对照母亲之间以及GDM后代和对照后代之间Pro/Ala基因型频率也显示相同结果（5.5%对9.2%，P>0.05；2.5%对3.0%，P>0.05）。（2）在GDM组和对照组中，Pro/Pro基因型孕妇的母亲FBS和各种脂质浓度与Pro/Ala基因型母亲相比均无显著差异（P>0.05）。（3）基于母亲和胎儿之间四种可能的PPARG2基因型配对，即Pro/Pro母亲及其Pro/Pro胎儿、Pro/Ala母亲及其Pro/Ala胎儿、Pro/Ala母亲及其Pro/Pro胎儿以及Pro/Pro母亲及其Pro/Ala胎儿，GDM组中Pro/Pro配对比对照组少，Pro/Ala配对比对照组多（72.5%对92.0%，P = 0.014；27.5%对6.0%，P<0.05）。
母亲和后代的Pro/Ala基因型均与GDM的发生无关。然而，母亲与其胎儿之间PPARG2 Pro12Ala多态性的差异暗示了GDM的一种可能原因。
