Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 4th Medical Department, Hanusch Hospital, Vienna, Austria.
Bone. 2010 Aug;47(2):392-9. doi: 10.1016/j.bone.2010.04.608. Epub 2010 May 5.
Important aspects of bone tissue quality include the physicochemical properties of its main constituents, the organic matrix and the mineral crystals. One of the most commonly reported measurements of Raman analysis of bone is the mineral to matrix ratio, obtained from the ratio of the integrated areas of any of the phosphate and amide peaks which depend on both tissue organization and composition. Cube-like samples of normal mouse cortical bone taken from the diaphysis and metaphysis of the femur were investigated within different age groups (2, 4, 8 and 12 weeks) by Raman microspectroscopy. Anatomically identical bone in both longitudinal and transverse directions was analyzed, enabling the discrimination between orientation and composition changes both as a function of animal age, and tissue age within the same animal. The results of the present study indicate that there is a parallel evolution of both orientation and chemical composition as a function of animal age, as well as tissue age within the same specimen. Our tissue age modified ratio of the carbonate to phosphate Raman peaks suggests that the bone mineral crystallite maturity remains relatively constant with animal age. Comparisons of polarized and depolarized experiments in the transversal plane of the diaphysis show a lack of orientation effects as a function of tissue age within the same animal, but exhibit differences as a function of animal age. In the metaphysis, the orientation effect is evident too, albeit less pronounced. This is most likely due to either the age difference between the two tissues within the same specimen in the long bone axis, as metaphyseal bone is generally younger than diaphyseal, or the more random orientation of the tissue collagen itself.
骨组织质量的重要方面包括其主要成分（有机基质和矿物质晶体）的物理化学性质。骨组织的拉曼分析中最常报告的测量之一是矿物质与基质的比率，该比率是通过依赖于组织组织和组成的任何磷酸盐和酰胺峰的积分面积之比获得的。使用拉曼微光谱法对来自股骨骨干和干骺端的正常小鼠皮质骨的立方状样本在不同年龄组（2、4、8 和 12 周）进行了研究。对在纵向上和横向上具有相同解剖结构的骨进行了分析，从而能够根据动物年龄和同一动物内组织的年龄来区分取向和组成变化。本研究的结果表明，随着动物年龄的增长以及同一标本内组织的年龄的增长，取向和化学组成都呈现出平行的演变。我们对骨矿物质中碳酸盐与磷酸盐拉曼峰的比值进行了组织年龄修正，结果表明骨矿物质微晶的成熟度相对保持不变。对骨干横截面上的偏振和非偏振实验的比较表明，在同一动物的组织年龄内，没有取向效应，但随着动物年龄的增长而存在差异。在干骺端，也存在取向效应，尽管不太明显。这很可能是由于同一标本中长骨轴上两个组织之间的年龄差异，因为骺端骨通常比骨干年轻，或者组织胶原本身的取向更随机。
