Dehring Karen A, Crane Nicole J, Smukler Abigail R, McHugh Jonathan B, Roessler Blake J, Morris Michael D
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Appl Spectrosc. 2006 Oct;60(10):1134-41. doi: 10.1366/000370206778664743.
Application of Raman spectroscopy to analysis of subchondral bone is described. The effect of cartilage health on subchondral bone has been widely studied using radiological and histological methods; however, there is no method to directly assay mineral components. We present Raman spectra of femur condyles and observe mineral bands that arise from the subchondral bone. In two separate experiments, transgenic mouse models of early-onset osteoarthritis (OA) and lipoatrophy were compared to tissue from wild-type mice. Raman spectroscopy was used to identify chemical changes in the mineral of subchondral bone that may accompany or precede morphological changes that can be observed by histology. The transgenic mice were compared to age-matched wild-type mice. Subtle alterations in the mineral or collagen matrix were observed by Raman spectroscopy using established Raman markers such as the carbonate-to-phosphate ratio, mineral-to-matrix ratio (MTMR), and amide I ratio. The Raman microscope configuration enabled rapid collection of Raman spectra from the mineralized layer that lies under an intact layer of non-mineralized articular cartilage. The effect of the cartilage layer on collection of spectra is discussed. The technique proposed is capable of providing insight into the chemical changes that occur in subchondral bone on a molecular level.
本文描述了拉曼光谱在软骨下骨分析中的应用。利用放射学和组织学方法，软骨健康对软骨下骨的影响已得到广泛研究；然而，尚无直接检测矿物质成分的方法。我们展示了股骨髁的拉曼光谱，并观察到来自软骨下骨的矿物质谱带。在两个独立实验中，将早发性骨关节炎（OA）和脂肪萎缩的转基因小鼠模型与野生型小鼠的组织进行了比较。拉曼光谱用于识别软骨下骨矿物质中可能伴随或先于组织学可观察到的形态学变化的化学变化。将转基因小鼠与年龄匹配的野生型小鼠进行比较。使用已确立的拉曼标记物，如碳酸盐与磷酸盐比率、矿物质与基质比率（MTMR）和酰胺I比率，通过拉曼光谱观察到矿物质或胶原基质的细微变化。拉曼显微镜配置能够从完整的非矿化关节软骨层下方的矿化层快速收集拉曼光谱。讨论了软骨层对光谱收集的影响。所提出的技术能够在分子水平上深入了解软骨下骨中发生的化学变化。
