Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
Front Endocrinol (Lausanne). 2023 May 8;14:1099889. doi: 10.3389/fendo.2023.1099889. eCollection 2023.
With the frequent use of Bisphosphonates (BPs), the morbidity of BP-related osteonecrosis of the jaw (BRONJ) is also increasing. However, the prevention and treatment of BRONJ is faced with enormous challenges. This study aimed to illuminate the influence of BP administration in the rat mandible and explore the feasibility of discriminating BRONJ lesion bone with Raman spectroscopy.
First, we explored the time- and mode-dependent effects of BP administration on the rat mandible with Raman spectroscopy. Second, the BRONJ rat model was constructed, and the lesion and healthy bone components were analyzed using Raman spectroscopy.
When only BPs were administered, no rats showed BRONJ symptoms, and no difference could be found in the Raman spectra. However, when combined with local surgery, six (6/8) rats showed BRONJ symptoms. The Raman spectra also showed a significant difference between the lesion and healthy bone.
In the progression of BRONJ, BPs and local stimulation play an essential role. Both BPs administration and local stimulation need to be controlled to prevent BRONJ. Moreover, BRONJ lesion bone in rats could be discriminated with Raman spectroscopy. This novel method would become a complement in the treatment of BRONJ in the future.
随着双膦酸盐（BPs）的频繁使用，BP 相关性下颌骨坏死（BRONJ）的发病率也在增加。然而，BRONJ 的预防和治疗面临着巨大的挑战。本研究旨在阐明 BP 给药在下颌骨中的时间和模式依赖性影响，并探索使用拉曼光谱区分 BRONJ 病变骨的可行性。
首先，我们使用拉曼光谱探索了 BP 给药对大鼠下颌骨的时间和模式依赖性影响。其次，构建了 BRONJ 大鼠模型，并使用拉曼光谱分析了病变和健康骨成分。
仅给予 BPs 时，没有大鼠出现 BRONJ 症状，拉曼光谱也没有差异。然而，当与局部手术联合使用时，有 6/8 只大鼠出现 BRONJ 症状。拉曼光谱也显示病变骨和健康骨之间存在显著差异。
在 BRONJ 的进展过程中，BPs 和局部刺激起着重要作用。需要控制 BPs 给药和局部刺激，以预防 BRONJ。此外，拉曼光谱可用于区分大鼠的 BRONJ 病变骨。这种新方法将成为未来 BRONJ 治疗的补充手段。
