Roszinski S, Wiedemann G, Jiang S Z, Baretton G, Wagner T, Weiss C
Department of Physiology, Med. Universität Lübeck, F.R.G.
Int J Radiat Oncol Biol Phys. 1991 Jun;20(6):1273-80. doi: 10.1016/0360-3016(91)90238-y.
The heat-induced interdependent changes of tumor blood flow, pO2, and pH decisively influence the therapeutic effect of hyperthermia (HT). This fact has induced us to determine simultaneously the frequency distribution of local pO2 values and the intratumoral pH in a xenotransplanted human sarcoma cell line at a normal blood glucose level and under hyperglycemic conditions before, during, and after HT. Two groups, one of 10 and one of 9 congenitally athymic nude rats with a subcutaneously implanted S117 human sarcoma into the right hind paw (mean tumor volume 5.3 cm3) were treated with local waterbath HT (tumor temperature 43 degrees C, 1 hr) alone or in combination with i.p. glucose injections (6 g/kg, 2 hr before the onset of HT). Tumor oxygenation remained improved throughout HT. Tumor pH did not decrease during HT. Hyperglycemia alone elicited a decrease of intratumoral pH and pO2, probably mainly due to hemoconcentration. The additional warming of the tumors (43 degrees C) during hyperglycaemia did not further decrease pO2 and pH. Silver stained sections of the tumors showed only a few very small necrotic areas, even in tumors of volumes up to 8 cm3. Our results indicate a well oxygenated tumor. In contrast to most tumors studied so far, hyperthermia in this tumor induces not only an initial increase of oxygenation but a lasting elevation of mean tumor pO2 for the duration of HT (up to 60 min).
热诱导的肿瘤血流、pO2和pH的相互依存变化对热疗（HT）的治疗效果具有决定性影响。这一事实促使我们在正常血糖水平和高血糖条件下，在热疗前、热疗期间和热疗后，同时测定异种移植的人肉瘤细胞系中局部pO2值的频率分布和瘤内pH值。将两组动物，一组10只和一组9只先天性无胸腺裸鼠，右后爪皮下植入S117人肉瘤（平均肿瘤体积5.3 cm3），分别单独进行局部水浴热疗（肿瘤温度43℃，1小时）或与腹腔注射葡萄糖（6 g/kg，热疗开始前2小时）联合治疗。在整个热疗过程中肿瘤氧合持续改善。热疗期间肿瘤pH值未降低。单独高血糖引起瘤内pH值和pO2降低，可能主要是由于血液浓缩。高血糖期间肿瘤额外升温（43℃）并未进一步降低pO2和pH值。肿瘤的银染切片显示仅有少数非常小的坏死区域，即使在体积达8 cm3的肿瘤中也是如此。我们的结果表明肿瘤氧合良好。与迄今为止研究的大多数肿瘤不同，该肿瘤的热疗不仅引起氧合的初始增加，而且在热疗持续期间（长达60分钟）平均肿瘤pO2持续升高。
