Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Biol Res Nurs. 2010 Jul;12(1):47-53. doi: 10.1177/1099800410366301. Epub 2010 May 7.
We evaluated the effects of sleep restriction on leptin levels in a large, diverse sample of healthy participants, while allowing free access to food.
Prospective experimental design. After 2 nights of baseline sleep, 136 participants (49% women, 56% African Americans) received 5 consecutive nights of 4 hours time in bed (TIB). Additionally, one subset of participants received 2 additional nights of either further sleep restriction (n = 27) or increased sleep opportunity (n = 37). Control participants (n = 9) received 10 hr TIB on all study nights. Plasma leptin was measured between 10:30 a.m. and 12:00 noon following baseline sleep, after the initial sleep-restriction period, and after 2 nights of further sleep restriction or recovery sleep.
Leptin levels increased significantly among sleep-restricted participants after 5 nights of 4 hr TIB (Z = -8.43, p < .001). Increases were significantly greater among women compared to men (Z = -4.77, p < .001) and among participants with higher body mass index (BMI) compared to those with lower (Z = -2.09, p = .036), though participants in all categories (sex, race/ethnicity, BMI, and age) demonstrated significant increases. There was also a significant effect of allowed TIB on leptin levels following the 2 additional nights of sleep restriction (p < .001). Participants in the control condition showed no significant changes in leptin levels.
These findings suggest that sleep restriction with ad libitum access to food significantly increases morning plasma leptin levels, particularly among women.
我们评估了在一个大型、多样化的健康参与者样本中，限制睡眠对瘦素水平的影响，同时允许自由进食。
前瞻性实验设计。在基线睡眠的 2 个晚上后，136 名参与者（49%为女性，56%为非裔美国人）接受了连续 5 个晚上的 4 小时卧床时间（TIB）。此外，一部分参与者接受了进一步的睡眠限制（n=27）或增加睡眠机会（n=37）的另外 2 个晚上。对照组参与者（n=9）在所有研究夜接受 10 小时 TIB。在基线睡眠后、初始睡眠限制期后以及进一步睡眠限制或恢复睡眠的 2 个晚上后，于上午 10:30 至 12:00 之间测量血浆瘦素。
在接受 4 小时 TIB 的 5 个晚上后，睡眠受限的参与者的瘦素水平显著升高（Z=-8.43，p<.001）。与男性相比，女性的增加更为显著（Z=-4.77，p<.001），与体重指数（BMI）较高的参与者相比，BMI 较低的参与者的增加更为显著（Z=-2.09，p=0.036），尽管所有类别的参与者（性别、种族/民族、BMI 和年龄）都显示出显著的增加。在进一步的 2 个晚上的睡眠限制后，允许的 TIB 对瘦素水平也有显著影响（p<.001）。对照组参与者的瘦素水平没有明显变化。
这些发现表明，限制睡眠并允许自由进食可显著增加早晨血浆瘦素水平，尤其是在女性中。
