Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, PR China.
Guangdong Institute of Microbiology, Guangdong Open Laboratory of Applied Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):816-822. doi: 10.1099/ijs.0.022228-0. Epub 2010 May 7.
Three aniline-degrading bacteria, strains DN316(T), DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged to the genus Rhizobium, with Rhizobium ( = Agrobacterium) radiobacter LMG 140(T) as the closest relative, with 96.5 % sequence similarity. Phylogenetic analysis of the representative strain DN316(T) using sequences of the glnA, thrC and recA genes and the 16S-23S intergenic spacer region confirmed the phylogenetic arrangement obtained from analysis of the 16S rRNA gene. DNA-DNA relatedness between DN316(T) and R. radiobacter LMG 140(T) was 43.7 %, clearly indicating that the representative strain DN316(T) represents a novel species. Phenotypic and biochemical characterization of the isolates and insertion sequence-PCR fingerprinting patterns showed several distinctive features that differentiated them from closely related species. The major components of the cellular fatty acids were C(18 : 1)ω7c (57.10 %), C(16 : 0) (11.31 %) and C(19 : 0) cyclo ω8c (10.13 %). Based on our taxonomic analysis, the three isolates from activated sludge represent a novel species of the genus Rhizobium, for which the name Rhizobium borbori sp. nov. is proposed. The type strain is DN316(T) ( = CICC 10378(T) = LMG 23925(T)).
从活性污泥中分离到三株苯胺降解菌,菌株 DN316(T)、DN316-1 和 DN365。根据 16S rRNA 基因序列的系统发育分析,这些分离物属于根瘤菌属,与 Rhizobium ( = Agrobacterium) radiobacter LMG 140(T)的亲缘关系最近,相似度为 96.5%。代表菌株 DN316(T)的系统发育分析使用 glnA、thrC 和 recA 基因序列和 16S-23S 间隔区序列证实了从 16S rRNA 基因分析中获得的系统发育排列。DN316(T)与 R. radiobacter LMG 140(T)之间的 DNA-DNA 相关度为 43.7%,清楚地表明代表菌株 DN316(T)代表一个新种。分离物的表型和生化特性以及插入序列-PCR 指纹图谱显示出一些区别于密切相关种的特征。细胞脂肪酸的主要成分是 C(18 : 1)ω7c (57.10%)、C(16 : 0) (11.31%)和 C(19 : 0)环 ω8c (10.13%)。根据我们的分类学分析,从活性污泥中分离到的三株菌代表了根瘤菌属的一个新种,建议将其命名为 Rhizobium borbori sp. nov.。模式菌株为 DN316(T) ( = CICC 10378(T) = LMG 23925(T))。