Li Yi, Lei Xueqian, Xu Yanting, Zhu Hong, Xu Meiying, Fu Lijun, Zheng Wei, Zhang Jinli, Zheng Tianling
College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.
Key Lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, 361005, China.
Curr Microbiol. 2017 Jan;74(1):42-48. doi: 10.1007/s00284-016-1149-y. Epub 2016 Oct 22.
A Gram-stain-negative, aerobic bacterial strain, designated Y21, was isolated from surface lake water in Xiamen, Fujian Province of China. Growth was observed at temperatures from 4 to 37 °C, at salinities from 0 to 7.0 % and at pH from 6.0 to 10.0. Optimum growth was observed at 28 °C, at pH 7.0 and with 1.5-2.0 % (w/v) NaCl. The highest similarity of 16S rRNA gene sequence between strain Y21 and the other strains was 96.9 %. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain was a member of the genus Rhizobium, forming a distinct lineage with R. subbaraonis KCTC 23614. The dominant fatty acids were summed feature 8 (comprising C ω7c and/or C ω6c), C ω7c 11-methyl, which accounted for 78.1 %. The G+C content of the chromosomal DNA was 60.9 mol%. The predominant respiratory quinone was ubiquinone-10. The polar lipids of strain Y21 were found to consist of five unidentified phospholipids and three unidentified aminolipids. According to its morphology, physiology, fatty acid composition and 16S rRNA sequence data, strain Y21 should be regarded as a new species of the genus Rhizobium, for which Rhizobium albus sp. nov. is proposed (type strain Y21 = MCCC 1F01210 = KCTC 42252).
从中国福建省厦门市的地表湖水中分离出一株革兰氏阴性需氧细菌菌株，命名为Y21。该菌株在4至37°C的温度、0至7.0%的盐度以及pH值为6.0至10.0的条件下均可生长。在28°C、pH值为7.0以及1.5 - 2.0%（w/v）NaCl的条件下观察到最佳生长状态。菌株Y21与其他菌株之间16S rRNA基因序列的最高相似性为96.9%。基于16S rRNA基因测序的系统发育分析表明，该菌株是根瘤菌属的成员，与根瘤菌KCTC 23614形成一个独特的谱系。主要脂肪酸为总特征8（包含C ω7c和/或C ω6c）、C ω
