Nemoto Manabu, Hata Hideaki, Higuchi Tohru, Imagawa Hiroshi, Yamanaka Takashi, Niwa Hidekazu, Bannai Hiroshi, Tsujimura Koji, Kondo Takashi, Matsumura Tomio
Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
J Vet Med Sci. 2010 Sep;72(9):1247-50. doi: 10.1292/jvms.10-0064. Epub 2010 Apr 28.
We evaluated antigen detection kits for human rotavirus with regard to their usefulness for diagnosing equine rotavirus infection. Limiting dilution analyses showed that of the seven kits investigated the Dipstick `Eiken' Rota (Dipstick) had the highest sensitivity to two serotypes of equine rotavirus. The Dipstick did not cross-react with several equine intestinal pathogens. An investigation using 249 fecal samples indicated that the sensitivity of the Dipstick was 81.9% and 47.3%, and its specificity was 98.2% and 99.0%, and its concordance rate was 92.8% and 68.3%, compared with values obtained using reverse transcription polymerase chain reaction and reverse transcription loop-mediated isothermal amplification, respectively. Although a negative result does not preclude the possibility of equine rotavirus infection, the Dipstick would be useful as routine test for diagnosing equine rotavirus infection in daily clinical practice because of its ease of handling.
我们评估了用于检测人轮状病毒的抗原检测试剂盒对诊断马轮状病毒感染的有效性。有限稀释分析表明，在所研究的7种试剂盒中，“荣研”试纸条法轮状病毒检测试剂盒（Dipstick）对两种马轮状病毒血清型的敏感性最高。该试纸条法检测试剂盒与几种马肠道病原体无交叉反应。一项对249份粪便样本的调查表明，与分别使用逆转录聚合酶链反应和逆转录环介导等温扩增法获得的值相比，该试纸条法检测试剂盒的敏感性分别为81.9%和47.3%，特异性分别为98.2%和99.0%，符合率分别为92.8%和68.3%。虽然阴性结果不能排除马轮状病毒感染的可能性，但由于操作简便，该试纸条法检测试剂盒在日常临床实践中作为诊断马轮状病毒感染的常规检测方法将很有用。
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