Conner M E, Gillespie J H, Schiff E I, Frey M S
Cornell Vet. 1983 Jul;73(3):280-7.
A total of 142 equine fecal samples (93 field fecal and 49 experimental fecal specimens) were examined for rotavirus using direct electron microscopy (EM) and the Rotazyme test. Eighty-six stool specimens were diarrhea samples. The Rotazyme test sensitivity and accuracy as compared to EM was determined by the visual (color reaction) and spectrophotometric methods. The overall agreement was 94.8% and 92.3% between EM and Rotazyme visual and spectrophotometric methods, respectively when suspect reactions (1 + color reaction or net absorbance between 0.05 and 0.1) were not included. The Rotazyme test is a quick, simple, and accurate diagnostic test for detection of rotavirus in equine fecal samples. It could be used by the equine practitioner with a minimum of laboratory facilities and by diagnostic and research laboratories.
共采集了142份马粪便样本（93份野外粪便样本和49份实验粪便样本），采用直接电子显微镜（EM）和Rotazyme检测法检测轮状病毒。86份粪便样本为腹泻样本。通过肉眼观察（颜色反应）和分光光度法确定Rotazyme检测法相对于EM的灵敏度和准确性。当不包括可疑反应（1+颜色反应或净吸光度在0.05至0.1之间）时，EM与Rotazyme肉眼观察法和分光光度法的总体一致性分别为94.8%和92.3%。Rotazyme检测法是一种快速、简单且准确的诊断方法，用于检测马粪便样本中的轮状病毒。它可供配备最少实验室设施的马医以及诊断和研究实验室使用。
