Mankin A S, Garrett R A
Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
J Bacteriol. 1991 Jun;173(11):3559-63. doi: 10.1128/jb.173.11.3559-3563.1991.
A broad range of antibiotics affecting protein biosynthesis were screened for their ability to inhibit growth of the archaeon Halobacterium halobium. Only a few drugs, including chloramphenicol, produced inhibitory effects. Mutants which showed increased resistance to chloramphenicol were isolated; of the nine tested, eight exhibited a C----U transition at position 2471 and the ninth had an A----C transversion at position 2088 of 23S rRNA. A double mutant containing both C----U (position 2471) and A----C (position 2088) mutations was isolated, but the level of its chloramphenicol resistance did not exceed that of either single-point mutant. Inferences are made concerning the functional significance of the conserved nucleotides in rRNAs.
对一系列影响蛋白质生物合成的抗生素进行了筛选，以考察它们抑制嗜盐古菌嗜盐栖热菌生长的能力。只有少数几种药物，包括氯霉素，产生了抑制作用。分离出了对氯霉素耐药性增强的突变体；在测试的9个突变体中，8个在23S rRNA的第2471位发生了C→U转换，第9个在第2088位发生了A→C颠换。分离出了一个同时含有C→U（第2471位）和A→C（第2088位）突变的双突变体，但其氯霉素耐药水平并未超过任何一个单点突变体。由此对rRNA中保守核苷酸的功能意义进行了推断。
