Clinical Microbiology Service, NewYork-Presbyterian Hospital, Columbia University Medical Center, New York, NY 10032, USA.
Can J Microbiol. 2010 Apr;56(4):326-32. doi: 10.1139/w10-016.
Rapid and accurate identification of Streptococcus pneumoniae is a critical component in the optimal management of infected patients. The performance of the BD Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, Md.) was evaluated for identification of S. pneumoniae (n = 311) and was compared to the Vitek 2 (bioMérieux, Marcy l'Etoile, France). Strains with discordant identification between methods were resolved with 16S rRNA gene sequencing as the gold standard. The Phoenix and the Vitek 2 correctly identified 96.8% (n = 301) and 95.2% (n = 296) of S. pneumoniae strains, respectively. Overall, there was no statistically significant difference in the performance of the 2 automated systems for the identification of S. pneumoniae in this study. The Vitek 2 mean time-to-results for all streptococcal identification was 1.5 h faster than that for the Phoenix. We conclude that the automated Phoenix and the Vitek 2 systems are comparable in their ability to identify S. pneumoniae and are preferable to the use of routine biochemical assays, which have delayed time-to-results and are not dependably accurate.
快速准确地鉴定肺炎链球菌是优化感染患者管理的关键组成部分。评估了 BD Phoenix 自动化微生物系统（BD 诊断系统，马里兰州斯帕克斯）对肺炎链球菌（n = 311）的鉴定性能，并与 Vitek 2（生物梅里埃，法国马西勒埃托勒）进行了比较。使用 16S rRNA 基因测序作为金标准来解决方法之间鉴定不一致的菌株。Phoenix 和 Vitek 2 分别正确鉴定了 96.8%（n = 301）和 95.2%（n = 296）的肺炎链球菌菌株。总体而言，在本研究中，这两种自动化系统在鉴定肺炎链球菌方面的性能没有统计学上的显著差异。Vitek 2 所有链球菌鉴定的平均结果时间比 Phoenix 快 1.5 小时。我们得出结论，自动化 Phoenix 和 Vitek 2 系统在鉴定肺炎链球菌方面具有相当的能力，优于常规生化检测方法，后者结果时间延迟且准确性不可靠。
