Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.
PLoS One. 2010 May 4;5(5):e10450. doi: 10.1371/journal.pone.0010450.
Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis.
Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle).
The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA).
纤毛室管膜细胞排列在脑室内，将感染的脑脊液与脑膜炎中的脑实质分隔开来。
研究李斯特菌与培养的大鼠脑室管膜细胞的相互作用表明，某些菌株降低了纤毛的摆动频率，但所有研究的菌株均显著降低了纤毛的摆动幅度（每个纤毛在一个摆动周期中移动的线性距离）。
室管膜的存在导致了某些李斯特菌菌株的聚集，在某些情况下，还可以看到细胞外物质与细菌聚集物相关联。这些观察结果依赖于侵袭、细胞内存活和李斯特菌细胞间传播所需基因的表达，这些基因的表达受转录激活因子、正调控因子 A（PrfA）调控。
