Regional Institute of Applied Scientific Research, Universidad de Castilla-La Mancha, Ciudad Real, Spain.
Appl Biochem Biotechnol. 2010 Nov;162(7):2058-66. doi: 10.1007/s12010-010-8981-5. Epub 2010 May 9.
Sixteen amylolytic yeasts, drawn from culture collections or isolated from different environments, were used in the present study. Experiments were performed to evaluate the starch degradation due to exocellular activity. The culture media of growth was optimized to find the maximum activity, and some strains produced an extracellular amylolytic enzyme when cultured in presence of starch in the medium. On the other hand, one yeast showed activity in cell extract when cultured under varying conditions in a bench bioreactor; this strain would thus be suitable for use as an animal feed supplement, both as a source of proteins and nucleic acids and in terms of its amylolytic activity.
在本研究中,使用了 16 株来自不同环境的淀粉分解酵母,从培养物中分离出来。进行了实验以评估由于细胞外活性引起的淀粉降解。优化了生长培养基以找到最大活性,并且一些菌株在培养基中存在淀粉时培养时会产生细胞外淀粉酶。另一方面,当在台式生物反应器中在不同条件下培养时,一种酵母在细胞提取物中显示出活性;因此,该菌株可作为动物饲料补充剂使用,无论是作为蛋白质和核酸的来源,还是从其淀粉分解活性方面来看,都是如此。