Department of Orthopaedic and Trauma Surgery, University of Oulu, Oulu, Finland.
Int Orthop. 2011 Apr;35(4):615-22. doi: 10.1007/s00264-010-1034-4. Epub 2010 May 9.
Bone extract from reindeer induces new ectopic bone formation (BF) in muscle pouches, but its feasibility in experimental bone lesions has not been evaluated. We investigated the effects of implants, containing 2, 5, 15, 20 or 50 mg of reindeer bone extract in a collagen carrier, on the healing of 8-mm femur defects in 78 rats. We used 30 µg of recombinant human bone morphogenetic protein-2 (rhBMP-2) in a collagen carrier, collagen and untreated defects as controls. Bone healing was evaluated with radiographs, peripheral quantitative computed tomography (pQCT), biomechanics and histology. In comparison with empty defects, the groups receiving bone extracts showed more BF at three weeks and had better bone union (BU), larger mean cross-sectional bone area at the defect site in groups receiving higher doses of extract, showed greater torsional stiffness of the bones and higher maximum breaking load of bones at six weeks. In comparison to all other groups, in the rhBMP-2 group, BF and BU were best at the three- and six-week follow-up, bone area was largest and mechanical test results were best. Although rhBMP-2 is superior for new bone regeneration, native reindeer bone extract is also effective in the six-week follow-up period.
从驯鹿中提取的骨提取物可在肌肉囊中诱导新的异位骨形成 (BF)，但其在实验性骨损伤中的可行性尚未得到评估。我们研究了植入物（含有 2、5、15、20 或 50mg 驯鹿骨提取物的胶原载体）对 78 只大鼠 8mm 股骨缺损愈合的影响。我们使用了 30μg 重组人骨形态发生蛋白-2（rhBMP-2）的胶原载体、胶原和未处理的缺陷作为对照。使用 X 射线、外周定量计算机断层扫描 (pQCT)、生物力学和组织学评估骨愈合。与空缺陷相比，在 3 周时，接受骨提取物的组显示出更多的 BF，并且具有更好的骨愈合 (BU)，在接受更高剂量提取物的组中，在缺陷部位的平均骨横截面积更大，显示出更大的骨骼扭转刚度和更高的骨骼最大断裂载荷。与所有其他组相比，在 rhBMP-2 组中，BF 和 BU 在 3 周和 6 周的随访中最佳，骨面积最大，力学测试结果最佳。虽然 rhBMP-2 更有利于新骨再生，但天然驯鹿骨提取物在 6 周的随访中也有效。
