Tölli Hanna, Birr Elli, Sandström Kenneth, Jämsä Timo, Jalovaara Pekka
Division of Orthopaedic and Trauma Surgery, Department of Surgery, Institute of Clinical Medicine, Oulu University Hospital, FIN-90014 University of Oulu, Finland.
Department of Medical Technology, Institute of Biomedicine, University of Oulu, FIN-90014 University of Oulu, Finland.
Materials (Basel). 2011 Jul 21;4(7):1321-1332. doi: 10.3390/ma4071321.
Various bone proteins and growth factors in specific concentrations are required for bone formation. If the body cannot produce sufficient quantities of these factors, bone trauma can be healed with an implant that includes the required factors in a carrier. This study was designed to evaluate various calcium salt candidates that can be used as carrier with reindeer bone protein extract to induce ectopic bone formation in the muscle pouch model of mouse. The bone protein extract was either impregnated into the disc form of carrier or mixed with carrier powder before implantation. The radiographic analysis indicated increased bone formation in all of the active groups containing the bone protein extract compared to the controls within 21 days follow-up. The highest bone formation was seen in the group with calcium sulfate with stearic acid where new bone and calcified cartilage were clearly visible. The greatest bone formation occurred in the groups that had bone protein extract readily available. This indicates that the bone forming factors in sufficient concentrations are required at the early stage of bone formation. The calcium sulfate with stearic acid was the most suitable and effective carrier for reindeer bone protein extract.
骨形成需要特定浓度的多种骨蛋白和生长因子。如果身体无法产生足够数量的这些因子，骨创伤可以通过植入物来愈合，该植入物在载体中包含所需因子。本研究旨在评估各种可用作载体的钙盐候选物，其与驯鹿骨蛋白提取物一起用于在小鼠肌肉袋模型中诱导异位骨形成。骨蛋白提取物要么浸渍到载体的圆盘形式中，要么在植入前与载体粉末混合。影像学分析表明，在21天的随访期内，与对照组相比，所有含有骨蛋白提取物的活性组中骨形成均增加。在含有硬脂酸硫酸钙的组中观察到最高的骨形成，其中新骨和钙化软骨清晰可见。最大的骨形成发生在骨蛋白提取物易于获得的组中。这表明在骨形成的早期阶段需要足够浓度的骨形成因子。硬脂酸硫酸钙是驯鹿骨蛋白提取物最合适和有效的载体。
