锂可预防镉对雄性大鼠睾丸的毒性作用。
Lithium protects against toxic effects of cadmium in the rat testes.
机构信息
Department of Obstetrics and Gynaecology, Faculty of Medicine, Kuwait University, Safat 13110, Kuwait.
出版信息
J Assist Reprod Genet. 2010 Aug;27(8):469-76. doi: 10.1007/s10815-010-9426-3. Epub 2010 May 9.
PURPOSE
To investigate the protective effect of Lithium against the toxic effect of Cadmium in the rat testes.
METHODS
Twenty four adult male Sprague-Dawley rats were treated with four different regimens: Cadmium only, Cadmium and lithium, lithium only and controls. Rats were sacrificed after 6 weeks and testicular levels of pro-inflammatory cytokine (IL-4), anti-inflammatory cytokine (TNF-α), Pro-apoptotic protein (Bax) and anti-apoptotic protein (Bcl-2) were measured by ELISA while serum levels of FSH, LH, Prolactin and Testosterone were measured using the Vidas parametric system. Antioxidant status (MDA, SOD) was also assessed in serum. Histopathological changes of testes were examined using light and electron microscopy. Immunohistochemical staining for Bax, Bcl-2 and Caspase 3 were performed.
RESULTS
Treatment with lithium was associated with significant reduction in the toxic effects of Cadmium as shown by reduced testicular levels of TNF-α, serum levels of Malondialdehyde and testicular level of Bax, and increased levels of IL-4, Zn-Cu SOD, Bcl-2 and Testosterone. Testicular histopathology showed that Cadmium produced an extensive germ cells apoptosis and the addition of lithium in Cadmium-treated rats significantly reduced cadmium-induced testicular damage.
CONCLUSION(S): Lithium has a protective effect against cadmium-induced testicular apoptosis in the rat.
目的
研究锂对大鼠睾丸镉毒性的保护作用。
方法
将 24 只成年雄性 Sprague-Dawley 大鼠分为四组：单独镉处理组、镉和锂联合处理组、单独锂处理组和对照组。6 周后处死大鼠，采用 ELISA 法检测睾丸组织中促炎细胞因子（IL-4）、抗炎细胞因子（TNF-α）、促凋亡蛋白（Bax）和抗凋亡蛋白（Bcl-2）的水平，采用 Vidas 参数系统检测血清中 FSH、LH、催乳素和睾酮的水平。同时还评估了血清中的抗氧化状态（MDA、SOD）。采用光镜和电镜观察睾丸的组织学变化。进行 Bax、Bcl-2 和 Caspase 3 的免疫组织化学染色。
结果
锂处理与镉的毒性作用的显著降低有关，表现为 TNF-α、血清丙二醛和睾丸 Bax 水平降低，IL-4、Zn-Cu SOD、Bcl-2 和睾酮水平升高。睾丸组织病理学显示，镉可引起广泛的生殖细胞凋亡，而在镉处理大鼠中加入锂可显著减轻镉引起的睾丸损伤。
结论
锂对大鼠镉诱导的睾丸细胞凋亡具有保护作用。
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