Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA.
Proteins. 2010 Jul;78(9):2170-5. doi: 10.1002/prot.22718.
The AT-rich interactive domain (ARID) of human AT-rich interactive domain-containing protein 3A (ARID3A) has been selected for structural characterization by Northeast Structural Genomics Consortium (residues 218-351 NESG ID HR4394C) as part of our Human Cancer Protein Interaction Network (HCPIN) project. Protein ARID3A belongs to the ARID family DNA-binding protein and is known to play important roles in embryonic patterning, cell lineage gene regulation, and cell cycle control, chromatin remodeling and transcriptional regulations. The solution NMR structure of ARID3A ARID domain consists of eight α-helices α0-α7 and a short β hairpin. Helix α0 and α1 form a V shape, helix α2-α4 and helix α5-α7 form two U shapes, and the V and two U shapes packed orthogonal to each other. The NMR structure of the ARID domain of human ARID3A reported here provides a structural basis for elucidating the regulatory mechanisms of ARID3A function, and the molecular mechanism of ARID3A interactions with DNA. It also has potential value in future drug discovery and design.
富含 AT 的相互作用结构域（ARID）的人类富含 AT 的相互作用结构域蛋白 3A（ARID3A）已被东北结构基因组学联盟（残基 218-351NESGIDHR4394C）选为结构特征选择，作为我们人类癌症蛋白相互作用网络（HCPIN）项目的一部分。蛋白 ARID3A 属于 ARID 家族 DNA 结合蛋白，已知在胚胎模式形成、细胞谱系基因调控和细胞周期控制、染色质重塑和转录调控中发挥重要作用。ARID3A 的 ARID 结构域的溶液 NMR 结构由 8 个α-螺旋α0-α7 和一个短的β发夹组成。螺旋α0 和α1 形成 V 形，螺旋α2-α4 和螺旋α5-α7 形成两个 U 形，V 形和两个 U 形相互垂直包装。这里报道的人 ARID3A 的 ARID 结构域的 NMR 结构为阐明 ARID3A 功能的调节机制以及 ARID3A 与 DNA 相互作用的分子机制提供了结构基础。它在未来的药物发现和设计中也具有潜在的价值。
