Delialioğlu Nuran, Otağ Feza, Ocal N Didem, Aslan Gönül, Emekda Görol
Mersin Universitesi Tip Fakuiltesi, Tibbi Mikrobiyoloji Anabilim Dali, Mersin.
Mikrobiyol Bul. 2010 Jan;44(1):87-91.
Aflatoxin M1 (AFM1) which is produced by some Aspergillus species, mainly Aspergillus flavus, is the hydroxylated metabolite of aflatoxin B1 (AFB1) and can be found in milk and dairy products of livestock fed with contaminated feed. AFM1 is a potential threat to human health owing to its toxic and carcino- genic effects. This study was aimed to investigate the level of AFM1 in raw and ultra-high-temperature pasteurized (UHT) milk samples. A total of 137 milk specimens (39 from goats, 53 from cows, and 45 from UHT marketmilks) were included to the study, and AFM1 levels were analyzed by high performance liquid chromatography (HPLC; Aflaprep M immunoaffinity column; R-Biopharm Rhone Ltd., Glasgow) method. AFM1 positivity was detected in 14 (35.8%) goat milk samples, in 46 (86.7%) cow milk samples and 33 (73.3%) UHT milk samples, with the levels ranging from 0.0021 microg/l to 0.8666 microg/I in raw milk, and from 0.001 microg/I to 0.059 microg/I in UHT milk samples. In 10.2% (4/39) of goat milk, 73.5% (39/53) of cow milk, and 2.2% (1/45) of UHT milk samples, the toxin levels were found to be above the officially reasonable limit value (> 0.05 microg/l) recommended by Turkish Food Codex Regulation. Since AFM1 levels were found to be significantly high in the raw milk samples collected in Mersin province (located on Mediterranean part of Turkey), it was concluded that people dealing with feed, milk and dairy products should be informed about the importance of aflatoxins and the preventive measures to get protected from AFB1 and AFM1.
黄曲霉毒素M1（AFM1）由某些曲霉菌种产生，主要是黄曲霉，它是黄曲霉毒素B1（AFB1）的羟基化代谢产物，可在食用受污染饲料的家畜的牛奶和乳制品中发现。由于其毒性和致癌作用，AFM1对人类健康构成潜在威胁。本研究旨在调查生牛奶和超高温瞬时灭菌（UHT）牛奶样品中AFM1的含量水平。该研究共纳入了137份牛奶样本（39份来自山羊奶，53份来自牛奶，45份来自市售UHT牛奶），并采用高效液相色谱法（HPLC；Aflaprep M免疫亲和柱；R-Biopharm Rhone Ltd.，格拉斯哥）分析AFM1含量水平。在14份（35.8%）山羊奶样本、46份（86.7%）牛奶样本和33份（73.3%）UHT牛奶样本中检测到AFM1呈阳性，生牛奶中AFM1含量范围为0.0021微克/升至0.8666微克/升，UHT牛奶样本中为0.001微克/升至0.059微克/升。在10.2%（4/39）的山羊奶、73.5%（39/53）的牛奶和2.2%（1/45）的UHT牛奶样本中，毒素含量被发现高于土耳其食品法典规定推荐的官方合理限量值（>0.05微克/升）。由于在梅尔辛省（位于土耳其地中海地区）采集的生牛奶样本中发现AFM1含量显著偏高，因此得出结论，应告知处理饲料、牛奶和乳制品的人员黄曲霉毒素的重要性以及预防措施，以避免受到AFB1和AFM1的危害。
