Cancer Prevention Laboratory, Department of Horticulture, Colorado State University, Fort Collins, Colorado 80523, USA.
J Agric Food Chem. 2010 Jun 9;58(11):6697-705. doi: 10.1021/jf1007538.
Dry bean consumption has been reported to be associated with reduced risk for a number of chronic diseases including cancer. The extent to which these benefits are associated with primary versus secondary plant metabolites is not known. The work reported herein focuses on low molecular weight secondary metabolites and uses longevity extension of wild-type Caenorhabditis elegans nematodes as a surrogate marker for human health benefits. A modified Bligh and Dyer technique was used to extract freeze-dried bean, and the resulting fractions were evaluated for longevity extension and metabolite fingerprinting using ultra performance liquid chromatography-mass spectrometry (UPLC-MS). Dry bean extracts extended adult C. elegans lifespan by as much as 16%. Hydrophilic fractions increased lifespan, whereas the hydrophobic fraction induced longevity reduction. Metabolite fingerprinting revealed distinguishing spectral differences among the four chemical fractions evaluated and demonstrated that within each fraction chemical composition differed significantly based on dry bean genetic heritage.
干豆的消费与多种慢性疾病（包括癌症）风险降低有关。但这些益处与初级或次级植物代谢物有关的程度尚不清楚。本文所报道的工作主要集中在低分子量的次级代谢物上，并使用野生型秀丽隐杆线虫的寿命延长作为人类健康益处的替代标志物。采用改良的 Bligh 和 Dyer 技术提取冻干豆，然后使用超高效液相色谱-质谱联用技术（UPLC-MS）对提取物进行寿命延长和代谢产物指纹图谱分析。干豆提取物使成年秀丽隐杆线虫的寿命延长了多达 16%。亲水部分可延长寿命，而疏水部分则会导致寿命缩短。代谢产物指纹图谱显示，在所评估的四个化学部分之间存在明显的光谱差异，并表明在每个部分内，根据干豆的遗传背景，化学成分有显著差异。
