Department of Engineering Materials, Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield, S3 7HQ, UK.
Regen Med. 2010 May;5(3):395-401. doi: 10.2217/rme.10.16.
To assess the potential of electrospun poly(lactide-co-glycolide) membranes to provide a biodegradable cell carrier system for limbal epithelial cells.
MATERIAL & METHODS: 50:50 poly(lactide-co-glycolide) scaffolds were spun, sterilized and seeded with primary rabbit limbal epithelial cells. Cells were cultured on the scaffolds for 2 weeks and then examined by confocal microscopy, cryosectioning and scanning-electron microscopy. The tensile strength of scaffolds before and after annealing and sterilization was also studied.
The limbal cells had formed a continuous multilayer of cells on either side of the scaffold. Scaffolds with cells showed signs of the onset of degradation within 2 weeks in culture media at 37 degrees C. Scaffolds that were annealed resulted in a more brittle and stiff mat.
We suggest this carrier membrane could be used as a replacement for the human amniotic membrane in the treatment of limbal stem cell deficiency, lowering the risk of disease transmission to the patient.
评估电纺聚（乳酸-共-乙醇酸）膜在为角膜缘上皮细胞提供可生物降解的细胞载体系统方面的潜力。
将 50:50 的聚（乳酸-共-乙醇酸）支架纺丝、消毒并接种原代兔角膜缘上皮细胞。细胞在支架上培养 2 周，然后通过共聚焦显微镜、冷冻切片和扫描电子显微镜进行检查。还研究了支架在退火和消毒前后的拉伸强度。
角膜缘细胞在支架两侧形成了连续的多层细胞。在 37°C 的培养介质中，培养 2 周后，带有细胞的支架开始出现降解迹象。退火后的支架变得更加脆弱和僵硬。
我们认为这种载体膜可以替代人羊膜用于治疗角膜缘干细胞缺陷，降低疾病向患者传播的风险。
