Department of Oral Medicine and Surgery, School of Dentistry, Complutense University, Madrid, Spain.
Clin Implant Dent Relat Res. 2012 Jun;14(3):395-9. doi: 10.1111/j.1708-8208.2010.00271.x. Epub 2010 Apr 23.
The aim of this study was to assess the effect of local melatonin administration on bone osseointegration around implants in rabbit tibiae.
Ten female, 6-month-old New Zealand rabbits were randomly divided into two groups: the experimental group, where five rabbits were treated with local application of melatonin (3 mg) to implant sites when placed into the rabbit tibia, and the control group, those who where without additive materials. Four weeks later, animals were sacrificed; tibiae were dissected from soft tissues and fixed in buffered formaldehyde, and then included in methacrylate. Histological sections were performed to be studied under light microscopy and analyzed morphometrically to evaluate the amount of bone to implant contact (BIC), trabecular area density, and cortical area density. One-way analysis of variance test was used for statistical evaluation. p < .05 was considered to be significant.
Histological evaluation showed more trabecular reaction in the melatonin group. Morphometrical analysis showed a statistically significant increase in trabecular BIC in the melatonin group when compared with the control group (24.61% ± 2.87 vs 13.62% ± 1.44; p < .01). Cortical BIC was decreased in the melatonin group, without statistical significance (71.08 ± 3.63 vs 76.28 ± 2.57; p = 0.31). Trabecular area density was increased significantly in the melatonin group (8.68 ± 1.61 vs 4.02 ± 0.36; p < .05). Cortical area density was decreased significantly in the melatonin group (91.31 ± 1.6 vs 95.7 ± 0.5; p < .05).
Within the limitation of this animal study, local melatonin application at the time of implant placement might induce more trabecular bone at implant contact and higher trabecular area density.
本研究旨在评估局部给予褪黑素对兔胫骨内种植体周围骨整合的影响。
10 只 6 月龄雌性新西兰大白兔随机分为两组：实验组在将种植体放入兔胫骨时，局部给予 3mg 褪黑素处理；对照组无添加材料。4 周后，处死动物；从软组织中解剖胫骨，用缓冲甲醛固定，然后用甲基丙烯酸酯包埋。进行组织学切片，在光镜下观察，并进行形态计量学分析，以评估种植体-骨接触（BIC）、骨小梁面积密度和皮质面积密度。采用单因素方差分析进行统计学评价。p＜0.05 被认为具有统计学意义。
组织学评价显示褪黑素组有更多的小梁反应。形态计量学分析显示，与对照组相比，褪黑素组的小梁 BIC 有统计学显著增加（24.61%±2.87 比 13.62%±1.44；p＜0.01）。褪黑素组皮质 BIC 降低，但无统计学意义（71.08±3.63 比 76.28±2.57；p=0.31）。褪黑素组骨小梁面积密度显著增加（8.68±1.61 比 4.02±0.36；p＜0.05）。褪黑素组皮质面积密度显著降低（91.31±1.6 比 95.7±0.5；p＜0.05）。
在本动物研究的限制范围内，种植体放置时局部给予褪黑素可能会在种植体接触处诱导更多的小梁骨，并增加小梁面积密度。
