Merino José Joaquín, Cabaña-Muñoz María Eugenia, Toledano Gasca Adolfo, Garcimartín Alba, Benedí Juana, Camacho-Alonso Fabio, Parmigiani-Izquierdo José María
Departamento de Farmacología, Farmacognosia y Botánica, Facultad de Farmacia, Universidad Complutense de Madrid (U.C.M), c/Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
Centro CIROM, Centro de Implantología y Rehabilitación Oral Multidisciplinaria, 30001 Murcia, Spain.
J Clin Med. 2019 Sep 2;8(9):1368. doi: 10.3390/jcm8091368.
Titanium is the mean biocompatible metal found in dental titanium alloys (Ti-6Al-4V). The safety of certain dental biomaterial amalgams has been questioned in patients. The levels of several systemic cytokines (interleukin (IL)-1 beta, IL-4: pg/mL) and chemokines (monocyte chemoattractant protein-1 (MCP-1), soluble fractalkine (CX3CL1: pg/mL) were determined using ELISA and compared between these study groups. The study included 30 controls without dental materials (cont), 57 patients with long-term titanium dental implants plus amalgams (A + I group) as well as 55 patients with long-term dental amalgam alone (A group). All patients (except controls) have had dental titanium implants (Ti-6Al-4V) and/or amalgams for at least 10 years (average: 15 years). We evaluated whether systemic levels of cytokines/chemokines, kyn/L-trp ratio and aromatic amino acid levels (HPLC: mM/L, Phe, L-Trp, His, Treo) could be altered in patients with long-term dental titanium and/or amalgams. These systemic markers were evaluated in 142 patients. The A + I group had higher L-Kynurenine/L-Tryptophan ratios than patients with long-term dental amalgam fillings alone (A). In addition, levels of IL-1 Beta cytokine, CX3CL1 and MCP-1 chemokines were higher in the A + I group than in the A group (A). The increased L-kyn/L-trp ratio and MCP-1 and fractalkine receptor (CX3CR1) elevations could suggest enhanced chemotactic responses by these chemokines in the A + I group.
钛是牙科钛合金（Ti-6Al-4V）中具有生物相容性的主要金属。某些牙科生物材料汞合金在患者体内的安全性受到质疑。使用酶联免疫吸附测定法（ELISA）测定了几种全身细胞因子（白细胞介素（IL）-1β、IL-4：pg/mL）和趋化因子（单核细胞趋化蛋白-1（MCP-1）、可溶性分形趋化因子（CX3CL1：pg/mL））的水平，并在这些研究组之间进行了比较。该研究包括30名未使用牙科材料的对照组（cont）、57名长期使用钛牙种植体加汞合金的患者（A + I组）以及55名仅长期使用牙科汞合金的患者（A组）。所有患者（对照组除外）使用牙科钛种植体（Ti-6Al-4V）和/或汞合金至少10年（平均：15年）。我们评估了长期使用牙科钛和/或汞合金的患者全身细胞因子/趋化因子水平、犬尿氨酸/L-色氨酸比值和芳香族氨基酸水平（高效液相色谱法：mM/L，苯丙氨酸、L-色氨酸、组氨酸、苏糖）是否会发生改变。在142名患者中对这些全身标志物进行了评估。A + I组的L-犬尿氨酸/L-色氨酸比值高于仅长期使用牙科汞合金填充物的患者（A组）。此外，A + I组的IL-1β细胞因子、CX3CL1和MCP-1趋化因子水平高于A组（A组）。L-犬尿氨酸/L-色氨酸比值的升高以及MCP-1和分形趋化因子受体（CX3CR1）的升高可能表明A + I组中这些趋化因子的趋化反应增强。
