State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Plant Genomics and National Plant Gene Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
New Phytol. 2010 Jul;187(2):301-312. doi: 10.1111/j.1469-8137.2010.03275.x. Epub 2010 Apr 28.
In higher plants, the crosstalk between cold stress responses and reactive oxygen species (ROS) signaling is not well understood. *Two chilling-sensitive mutants, chs4-1 and chs4-3, were characterized genetically and molecularly. *The CHS4 gene, identified by map-based cloning, was found to be identical to lesion simulating disease resistance 1 (LSD1). We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid. These results indicate that low temperature is another trigger of cell death in lsd1 mutants. Furthermore, lsd1-3 plants accumulated higher concentrations of H(2)O(2) and total glutathione under cold conditions than wild-type plants. Genetic analysis revealed that PAD4 and EDS1, two key signaling regulators mediating resistance responses, are required for the chilling-sensitive phenotype of lsd1-3. *These findings reveal a role of LSD1 in regulating cell death trigged by cold stress and a link between cold stress responses and ROS-associated signaling.
在高等植物中，冷胁迫反应和活性氧（ROS）信号之间的串扰还没有得到很好的理解。我们对两个冷敏感突变体 chs4-1 和 chs4-3 进行了遗传和分子特征分析。通过图谱定位克隆鉴定出 CHS4 基因，发现它与病变模拟疾病抗性 1（LSD1）基因相同。因此，我们分别将这两个等位基因重新命名为 lsd1-3 和 lsd1-4。这两个突变体在冷胁迫条件下表现出广泛的细胞死亡表型。一致的是，冷胁迫下的 lsd1-3 植物表现出 PR1 和 PR2 基因的上调表达，以及水杨酸的积累增加。这些结果表明，低温是 lsd1 突变体细胞死亡的另一个触发因素。此外，与野生型植物相比，冷胁迫下的 lsd1-3 植物积累了更高浓度的 H2O2 和总谷胱甘肽。遗传分析表明，PAD4 和 EDS1 这两个介导抗性反应的关键信号调节因子，是 lsd1-3 冷敏感表型所必需的。这些发现揭示了 LSD1 在调节由冷应激触发的细胞死亡中的作用，以及冷应激反应与 ROS 相关信号之间的联系。
