Li Qiaolu, Hu Rongrong, Jiang Min, Zhang Wei, Gao Xinyi, Zhang Binglin, Liu Weijuan, Wu Zhongyi, Zou Huawen
College of Agriculture, Yangtze University, Jingzhou 434025, China.
Tianmen Academy of Agricultural Sciences, Tianmen 431700, China.
Plants (Basel). 2024 Oct 17;13(20):2904. doi: 10.3390/plants13202904.
Salt stress significantly impairs plant growth, presenting a challenge to agricultural productivity. Exploring the regulatory mechanisms underlying salt stress responses is critically important. Here, we identified a significant role for the maize LESION-SIMULATING DISEASE transcription factor, ZmLSD1, in enhancing salt stress response. Subcellular localization analysis indicated that ZmLSD1-GFP was localized in the nucleus in the maize protoplast. Overexpressing in maize obviously enhanced the tolerance of plants to salt stress. Physiological analysis indicated that overexpressed in maize could mitigate the accumulation of HO and MDA content exposed to salt stress. RNA-seq and qPCR-PCR analyses showed that ZmLSD1 positively regulated expression. ChIP-qPCR and EMSA experiments demonstrated that ZmLSD1 could directly bind to the promoter of through the GTAC motif both in vitro and in vivo. Overall, our findings suggest that ZmLSD1 plays a positive role in enhancing the tolerance of maize to salt by affecting expression.
盐胁迫显著损害植物生长，对农业生产力构成挑战。探索盐胁迫响应的调控机制至关重要。在此，我们确定了玉米损伤模拟病转录因子ZmLSD1在增强盐胁迫响应中的重要作用。亚细胞定位分析表明，ZmLSD1-GFP定位于玉米原生质体的细胞核中。在玉米中过表达明显增强了植物对盐胁迫的耐受性。生理分析表明，在玉米中过表达可以减轻盐胁迫下HO和MDA含量的积累。RNA-seq和qPCR-PCR分析表明，ZmLSD1正向调控表达。ChIP-qPCR和EMSA实验表明，ZmLSD1在体外和体内均可通过GTAC基序直接结合到的启动子上。总体而言，我们的研究结果表明，ZmLSD1通过影响表达在增强玉米对盐的耐受性方面发挥积极作用。
