Provost-Javier K N, Chen S, Rasgon J L
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
Insect Mol Biol. 2010 Aug;19(4):423-9. doi: 10.1111/j.1365-2583.2010.00999.x. Epub 2010 Apr 27.
Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but little is known about regulation of vitellogenesis in autogenous mosquitoes. We characterized the expression of four vitellogenin genes (Vg1a, Vg1b, Vg2a and Vg2b) in an autogenous strain of the West Nile Virus vector, Culex tarsalis. All vitellogenin genes were expressed during autogenous reproduction and following a bloodmeal, although the intensity and duration of expression varied amongst genes. Quantitative PCR analysis of vitellogenin transcription during autogeny revealed a similar temporal pattern to known vitellogenin expression profiles in anautogenous Aedes aegypti. Vitellogenin transcript, primarily produced from the Vg1b gene, was also detected in the larval and pupal stages of development, but no detectable vitellogenin protein was produced during this time period.
自体生殖，即蚊子无需吸食血液就能使第一批卵成熟的能力，是一种对病媒传播疾病传播具有重要影响的替代生殖策略。在血餐诱导的卵子发生过程中，主要卵黄蛋白（卵黄原蛋白；Vg）基因的调控已得到充分研究，但关于自体生殖蚊子中卵黄发生的调控却知之甚少。我们对西尼罗河病毒媒介致倦库蚊的一个自体生殖品系中的四个卵黄原蛋白基因（Vg1a、Vg1b、Vg2a和Vg2b）的表达进行了表征。所有卵黄原蛋白基因在自体生殖和血餐之后均有表达，尽管各基因的表达强度和持续时间有所不同。对自体生殖过程中卵黄原蛋白转录的定量PCR分析显示，其时间模式与非自体生殖的埃及伊蚊中已知的卵黄原蛋白表达谱相似。在发育的幼虫和蛹阶段也检测到了主要由Vg1b基因产生的卵黄原蛋白转录本，但在此时间段内未检测到可检测到的卵黄原蛋白。
