一种通过杂交瘤技术生产鸡单克隆抗体的新细胞系。

A new cell line for the production of chicken monoclonal antibody by hybridoma technology.

作者信息

Nishinaka S, Suzuki T, Matsuda H, Murata M

机构信息

Biotechnology Development Center, NKK Corporation, Kawasaki, Japan.

出版信息

J Immunol Methods. 1991 Jun 3;139(2):217-22. doi: 10.1016/0022-1759(91)90191-h.

DOI:10.1016/0022-1759(91)90191-h

PMID:2045661

Abstract

In our previous study (Nishinaka et al., 1989), a chicken B cell line (HU3R27) deficient in thymidine kinase activity was used as a fusion partner for production of chicken monoclonal antibody. Specific antibody-producing hybridomas were initially obtained, but they soon lost the ability to produce antibody in culture. The present report documents the development of an improved fusion cell line. R27H4, for the production of chicken monoclonal antibody. This cell line was obtained by fusion of HU3R27 cells with spleen cells of immunized chicken. Fusion of R27H4 cells with spleen cells from chicken immunized by keyhole limpet hemocyanin (KLH) resulted in antibody-producing hybridomas. Subcloned hybridomas secreted highly reactive IgG and weakly reactive IgM. Some of the cell lines have been displaying stable antibody secretions for over 6 months.

摘要

在我们之前的研究中（Nishinaka等人，1989年），一种缺乏胸苷激酶活性的鸡B细胞系（HU3R27）被用作生产鸡单克隆抗体的融合伙伴。最初获得了产生特异性抗体的杂交瘤，但它们很快就失去了在培养物中产生抗体的能力。本报告记录了一种改进的融合细胞系R27H4用于生产鸡单克隆抗体的研发过程。该细胞系是通过HU3R27细胞与免疫鸡的脾细胞融合获得的。R27H4细胞与经钥孔戚血蓝蛋白（KLH）免疫的鸡的脾细胞融合，产生了产生抗体的杂交瘤。亚克隆的杂交瘤分泌高反应性的IgG和低反应性的IgM。一些细胞系已经稳定分泌抗体超过6个月。