Grunow R, Jahn S, Porstmann T, Kiessig S S, Steinkellner H, Steindl F, Mattanovich D, Gürtler L, Deinhardt F, Katinger H
Department of Medicine (Charite), Humboldt University Berlin, G.D.R.
J Immunol Methods. 1988 Feb 10;106(2):257-65. doi: 10.1016/0022-1759(88)90206-2.
This paper describes the construction of a new heteromyeloma cell line designated CB-F7. The cell line was derived from xenogeneic somatic cell hybridization between normal human B lymphocytes and the murine HAT-sensitive P3X63Ag8/653 cell line. CB-F7 cells were characterized by rapid cell growth (doubling time about 16 h) and high cloning efficiencies in culture medium supplemented with 10% or 5% fetal calf serum, respectively. The karyotype of the cells consists of about 75-78 chromosomes as well as two chromosomal fragments. Fusions of the cells with human peripheral blood cells resulted in approximately 2-6 clones per 10(5) seeded lymphocytes. Furthermore, the cells are ouabain resistant and therefore suitable for fusions with EBV-transformed lymphoblastoid cell lines. Using CB-F7 as the parental cell line a number of specific human mAb producing hybrids were established. For the first time, we describe here the generation of hybrids secreting human monoclonal antibodies to human immunodeficiency virus (HIV). Two monoclonal antibodies of IgG type and one of IgM type reacted with the major core protein p25 and one IgG antibody reacted with the transmembrane protein gp41.
本文描述了一种新的异源骨髓瘤细胞系CB-F7的构建。该细胞系源自正常人B淋巴细胞与鼠源HAT敏感的P3X63Ag8/653细胞系之间的异种体细胞杂交。CB-F7细胞的特点是细胞生长迅速（倍增时间约16小时），在分别添加10%或5%胎牛血清的培养基中克隆效率高。细胞的核型由约75 - 78条染色体以及两个染色体片段组成。这些细胞与人外周血细胞融合后，每10⁵个接种的淋巴细胞可产生约2 - 6个克隆。此外，这些细胞对哇巴因具有抗性，因此适合与EB病毒转化的淋巴母细胞系融合。以CB-F7作为亲本细胞系，建立了许多产生特异性人单克隆抗体的杂交细胞系。我们首次在此描述了分泌针对人类免疫缺陷病毒（HIV）的人单克隆抗体的杂交细胞系的产生。两种IgG型单克隆抗体和一种IgM型单克隆抗体与主要核心蛋白p25反应，一种IgG抗体与跨膜蛋白gp41反应。
