State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Bioresour Technol. 2010 Sep;101(18):7104-10. doi: 10.1016/j.biortech.2010.03.129. Epub 2010 Apr 24.
Saccharomyces cerevisiae strain NAN-127 (2n, prototroph), which contains the xylose reductase-xylitol dehydrogenase (XR-XDH) xylose metabolic pathway was used for the cofermentation of glucose and xylose. Oxygen supply was the most important factor for xylose fermentation and pH 4.5 and a ventilation rate of 0.04 vvm were optimal. The xylose utilization ratio reached 0.655 at an initial xylose concentration of 50 gL(-1) and was 0.9 at an initial concentration of 20 gL(-1). Addition of furfural at late logarithmic phase as electron acceptor to a final concentration of 3.0 gL(-1) decreased the xylitol yield by 17% under micro-aeration conditions without inhibiting cell growth, but also without an increase in ethanol yield. The results are important to the application of strain NAN-127 in the lignocellulosic ethanol process.
酿酒酵母 NAN-127 菌株（2n，营养缺陷型），含有木糖还原酶-木糖醇脱氢酶（XR-XDH）木糖代谢途径，用于葡萄糖和木糖的共发酵。供氧是木糖发酵的最重要因素，pH 值 4.5 和通气率 0.04 vvm 是最佳条件。在初始木糖浓度为 50 g/L(-1)时，木糖利用率达到 0.655，初始浓度为 20 g/L(-1)时达到 0.9。在对数后期向微曝气条件下添加终浓度为 3.0 g/L(-1)的糠醛作为电子受体，不会抑制细胞生长，但也不会增加乙醇产量，从而使木糖醇产量降低 17%。这些结果对酿酒酵母 NAN-127 在木质纤维素乙醇工艺中的应用非常重要。
