Institute for Environmental Protection and Research (ISPRA), Laboratory of Genetics, Via Cà Fornacetta 9, 40064 Ozzano dell'Emilia (BO), Italy.
Forensic Sci Int Genet. 2010 Oct;4(5):334-8. doi: 10.1016/j.fsigen.2009.10.012. Epub 2009 Nov 26.
The recent expansion of the Italian wolf population through the Apennine and western Alps, after centuries of contractions, is causing conflicts with human activities leading to a rise in poaching or illegal killings. Here we show how molecular population genetics has been used to identify a suspect serial wolf killer. We analysed DNA extracted from a necklace made of ten presumed wolf canine teeth, confiscated in 2008 to a man living in the northern Italian Apennine (Liguria Region). Individual genotypes were determined using 12 unlinked autosomal microsatellites (STRs), mtDNA control-region sequences, a male-specific ZFX/ZFY restriction-site and three Y-linked STRs. Results indicate that the teeth belonged to six different individuals (three males and three females), which were assigned to the Italian wolf population with p > 0.90 by Bayesian procedures. One of these genotypes matched with the genetic profile of a male wolf previously found-dead and already non-invasively sampled in the same area. Another genotype matched with that of a female wolf non-invasively sampled twice in the same area 1 year before. These data are being used as forensic genetic evidence in the ongoing criminal trial against the suspect serial wolf killer.
近年来，意大利狼群在经历了几个世纪的收缩后，通过亚平宁山脉和阿尔卑斯山西部扩张，导致与人类活动的冲突加剧，偷猎或非法杀戮事件有所增加。在这里，我们展示了分子群体遗传学如何用于识别一名涉嫌连环杀狼者。我们分析了从 2008 年一名居住在意大利北部亚平宁山脉（利古里亚地区）的男子身上没收的一串由十颗疑似狼犬齿制成的项链中的 DNA。使用 12 个不连锁的常染色体微卫星（STRs）、mtDNA 控制区序列、一个雄性特异性 ZFX/ZFY 限制位点和三个 Y 连锁 STRs 确定了个体基因型。结果表明，这些牙齿属于六个不同的个体（三个雄性和三个雌性），贝叶斯程序表明它们属于意大利狼群，概率大于 0.90。其中一个基因型与之前在同一地区发现死亡并已进行非侵入性采样的雄性狼的遗传特征相匹配。另一个基因型与一年前在同一地区两次进行非侵入性采样的雌性狼的遗传特征相匹配。这些数据正在作为正在进行的针对涉嫌连环杀狼者的刑事审判的法医遗传证据。
