Department of Chemistry, University of Auckland, New Zealand.
Forensic Sci Int Genet. 2011 Jan;5(1):64-8. doi: 10.1016/j.fsigen.2009.11.007. Epub 2010 Jan 6.
The use of messenger RNA profiling to identify the origin of biological samples (e.g. blood, semen and saliva) from crime scenes is now at the stage of being implemented into routine forensic casework. We report on the successful modification of the Promega DNA IQ™ system to enable co-extraction of DNA and RNA from the same sample without compromising the potential DNA profile. Using the protocol in our laboratory for extracting DNA using the DNA IQ™ system combined with the Zymo Research Mini RNA Isolation Kit™ II we demonstrate the simultaneous co-extraction of DNA and RNA from the same sample for routine DNA and mRNA profiling for the identification of both the individual and the biological stain.
信使 RNA 分析用于鉴定犯罪现场生物样本（如血液、精液和唾液）的来源，目前正处于常规法医案件工作的实施阶段。我们报告了成功修改 Promega DNA IQ™系统，使其能够在不影响潜在 DNA 图谱的情况下，从同一样本中同时提取 DNA 和 RNA。我们使用实验室中使用 DNA IQ™系统提取 DNA 的方案，结合 Zymo Research Mini RNA Isolation Kit™ II，证明了从同一样本中同时提取 DNA 和 RNA 进行常规 DNA 和 mRNA 分析，以鉴定个体和生物痕迹。
