Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
Chem Senses. 2010 Jul;35(6):501-9. doi: 10.1093/chemse/bjq040. Epub 2010 May 9.
A healthy taste system is important to the maintenance of nutrition and overall quality of life, and taste disorders are associated with many inflammatory states. We previously determined the immune cells in normal human gustatory tissue; they are predominantly dendritic cells and CD4 T cells with a few macrophages and B lymphocytes present. There are, however, few reports of the subtypes of resident lymphocytes in or near taste tissues. The present study further characterized the distribution and population of the major subtypes of T cells in situ within biopsies of healthy human fungiform papillae (FP). Immunohistochemical analyses indicated that T-helper (Th)1 cells (CCR5+) were more predominant in FP than Th2 T cells (CCR4+). CD45RO+ memory T cells were the principal T cells in gustatory tissue, whereas CD45RA+ naive T cells were uncommon. Regarding subcompartments of the tissue, most intraepithelial lymphocytes of FPs were gamma/delta T cells, whereas the major subtype of lymphocytes in the lamina propria were alpha/beta T cells. Regulatory T cells that express CTLA-4 (CD152) and interleukin-2 receptors (IL-2R, CD25) were found at low levels in FP. The T cells stand ready to respond to inflammatory and infectious insults and may play a role in the taste alterations observed during acute and chronic inflammatory states.
健康的味觉系统对于维持营养和整体生活质量很重要，而味觉障碍与许多炎症状态有关。我们之前确定了正常人类味觉组织中的免疫细胞；它们主要是树突状细胞和 CD4 T 细胞，少数巨噬细胞和 B 淋巴细胞存在。然而，关于味觉组织内或附近常驻淋巴细胞亚型的报道很少。本研究进一步描述了健康人菌状乳头活检标本中主要 T 细胞亚群的分布和群体。免疫组织化学分析表明，与 Th2 T 细胞（CCR4+）相比，辅助性 T 细胞（Th）1 细胞（CCR5+）在 FP 中更为普遍。CD45RO+记忆 T 细胞是味觉组织中的主要 T 细胞，而 CD45RA+幼稚 T 细胞则不常见。关于组织的亚区，大多数 FP 的上皮内淋巴细胞是γ/δ T 细胞，而固有层中的主要淋巴细胞亚型是α/β T 细胞。在 FP 中发现表达 CTLA-4（CD152）和白细胞介素-2 受体（IL-2R，CD25）的调节性 T 细胞水平较低。T 细胞随时准备对炎症和感染的侵袭做出反应，可能在急性和慢性炎症状态下观察到的味觉改变中发挥作用。
