• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种新型的单链DNA特异性3'-5'核酸外切酶，嗜热栖热菌核酸外切酶I，参与多种DNA修复途径。

A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways.

作者信息

Shimada Atsuhiro, Masui Ryoji, Nakagawa Noriko, Takahata Yoshio, Kim Kwang, Kuramitsu Seiki, Fukui Kenji

机构信息

Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043 and RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.

出版信息

Nucleic Acids Res. 2010 Sep;38(17):5692-705. doi: 10.1093/nar/gkq350. Epub 2010 May 10.

DOI:10.1093/nar/gkq350
PMID:20457749
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2943613/
Abstract

Single-stranded DNA (ssDNA)-specific exonucleases (ssExos) are expected to be involved in a variety of DNA repair pathways corresponding to their cleavage polarities; however, the relationship between the cleavage polarity and the respective DNA repair pathways is only partially understood. To understand the cellular function of ssExos in DNA repair better, genes encoding ssExos were disrupted in Thermus thermophilus HB8 that seems to have only a single set of 5'-3' and 3'-5' ssExos unlike other model organisms. Disruption of the tthb178 gene, which was expected to encode a 3'-5' ssExo, resulted in significant increase in the sensitivity to H(2)O(2) and frequency of the spontaneous mutation rate, but scarcely affected the sensitivity to ultraviolet (UV) irradiation. In contrast, disruption of the recJ gene, which encodes a 5'-3' ssExo, showed little effect on the sensitivity to H(2)O(2), but caused increased sensitivity to UV irradiation. In vitro characterization revealed that TTHB178 possessed 3'-5' ssExo activity that degraded ssDNAs containing deaminated and methylated bases, but not those containing oxidized bases or abasic sites. Consequently, we concluded that TTHB178 is a novel 3'-5' ssExo that functions in various DNA repair systems in cooperation with or independently of RecJ. We named TTHB178 as T. thermophilus exonuclease I.

摘要

单链DNA（ssDNA）特异性核酸外切酶（ssExos）有望参与与其切割极性相对应的多种DNA修复途径；然而，切割极性与各自DNA修复途径之间的关系仅得到部分理解。为了更好地理解ssExos在DNA修复中的细胞功能，在嗜热栖热菌HB8中破坏了编码ssExos的基因，与其他模式生物不同，嗜热栖热菌HB8似乎只有一组5'-3'和3'-5'ssExos。预期编码3'-5'ssExo的tthb178基因的破坏导致对H₂O₂的敏感性显著增加和自发突变率频率显著增加，但几乎不影响对紫外线（UV）照射的敏感性。相反，编码5'-3'ssExo的recJ基因的破坏对H₂O₂的敏感性几乎没有影响，但导致对紫外线照射的敏感性增加。体外特性分析表明，TTHB178具有3'-5'ssExo活性，可降解含有脱氨基和甲基化碱基的ssDNA，但不能降解含有氧化碱基或无碱基位点的ssDNA。因此，我们得出结论，TTHB178是一种新型的3'-5'ssExo，它在各种DNA修复系统中与RecJ协同或独立发挥作用。我们将TTHB178命名为嗜热栖热菌核酸外切酶I。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/082f7026223a/gkq350f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/cf4e990121cb/gkq350f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/ac204e5d92f4/gkq350f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/5b79bf942714/gkq350f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/b545c8269abe/gkq350f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/4618b65ff410/gkq350f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/bb1dbe1c5738/gkq350f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/1082cd7a30d3/gkq350f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/082f7026223a/gkq350f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/cf4e990121cb/gkq350f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/ac204e5d92f4/gkq350f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/5b79bf942714/gkq350f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/b545c8269abe/gkq350f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/4618b65ff410/gkq350f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/bb1dbe1c5738/gkq350f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/1082cd7a30d3/gkq350f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3672/2943613/082f7026223a/gkq350f8.jpg

相似文献

1
A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways.一种新型的单链DNA特异性3'-5'核酸外切酶，嗜热栖热菌核酸外切酶I，参与多种DNA修复途径。
Nucleic Acids Res. 2010 Sep;38(17):5692-705. doi: 10.1093/nar/gkq350. Epub 2010 May 10.
2
Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain.嗜热栖热菌HB8中RecJ蛋白及其核心结构域的过表达、纯化与特性分析
Nucleic Acids Res. 2001 Nov 15;29(22):4617-24. doi: 10.1093/nar/29.22.4617.
3
The crystal structure of exonuclease RecJ bound to Mn2+ ion suggests how its characteristic motifs are involved in exonuclease activity.与锰离子结合的核酸外切酶RecJ的晶体结构表明其特征基序是如何参与核酸外切酶活性的。
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5908-12. doi: 10.1073/pnas.092547099. Epub 2002 Apr 23.
4
Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidance.大肠杆菌中的单链DNA特异性核酸外切酶。在修复和避免突变中的作用。
Genetics. 1998 May;149(1):7-16. doi: 10.1093/genetics/149.1.7.
5
Structure of RecJ exonuclease defines its specificity for single-stranded DNA.RecJ 核酸外切酶的结构决定了其对单链 DNA 的特异性。
J Biol Chem. 2010 Mar 26;285(13):9762-9769. doi: 10.1074/jbc.M109.096487. Epub 2010 Feb 2.
6
Role of RecJ-like protein with 5'-3' exonuclease activity in oligo(deoxy)nucleotide degradation.RecJ 样蛋白 5'-3' 外切核酸酶活性在寡（脱氧）核苷酸降解中的作用。
J Biol Chem. 2011 Jan 28;286(4):2807-16. doi: 10.1074/jbc.M110.161596. Epub 2010 Nov 18.
7
Orchestration of Haemophilus influenzae RecJ exonuclease by interaction with single-stranded DNA-binding protein.通过与单链DNA结合蛋白相互作用对流感嗜血杆菌RecJ核酸外切酶进行调控。
J Mol Biol. 2009 Feb 6;385(5):1375-96. doi: 10.1016/j.jmb.2008.11.041. Epub 2008 Nov 27.
8
Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity.嗜热栖热菌HB8中DNA聚合酶X的特性揭示，POLXc和PHP结构域对于3'-5'核酸外切酶活性都是必需的。
Nucleic Acids Res. 2009 Apr;37(6):2037-52. doi: 10.1093/nar/gkp064. Epub 2009 Feb 11.
9
The biochemical characterization of a TatD nuclease from Thermus thermophilus.热栖热袍菌 TatD 核酸内切酶的生化特性研究。
Protein Expr Purif. 2024 Nov;223:106557. doi: 10.1016/j.pep.2024.106557. Epub 2024 Jul 14.
10
Neisseria gonorrhoeae recJ mutants show defects in recombinational repair of alkylated bases and UV-induced pyrimidine dimers.淋病奈瑟菌recJ突变体在烷基化碱基和紫外线诱导的嘧啶二聚体的重组修复中表现出缺陷。
Mol Gen Genet. 2000 Oct;264(3):268-75. doi: 10.1007/s004380000316.

引用本文的文献

1
Effect of methyl DNA adducts on 3'-5' exonuclease activity of human TREX1.甲基化DNA加合物对人TREX1 3'-5'核酸外切酶活性的影响。
Biochem J. 2025 Mar 5;482(5):263-273. doi: 10.1042/BCJ20240600.
2
A Plasmodium apicoplast-targeted unique exonuclease/FEN exhibits interspecies functional differences attributable to an insertion that alters DNA-binding.疟原虫质体靶向的独特核酸外切酶/FEN 表现出种间功能差异，这归因于一个插入突变导致 DNA 结合改变。
Nucleic Acids Res. 2024 Jul 22;52(13):7843-7862. doi: 10.1093/nar/gkae512.
3
Structural insights into the duplex DNA processing of TREX2.

本文引用的文献

1
Tandem mass spectrometry of small, multiply charged oligonucleotides.串联质谱法分析小的、多电荷寡核苷酸。
J Am Soc Mass Spectrom. 1992 Jan;3(1):60-70. doi: 10.1016/1044-0305(92)85019-G.
2
Transcription profile of Thermus thermophilus CRISPR systems after phage infection.嗜热栖热菌 CRISPR 系统在噬菌体感染后的转录谱。
J Mol Biol. 2010 Jan 15;395(2):270-81. doi: 10.1016/j.jmb.2009.10.057. Epub 2009 Nov 3.
3
Degradation of ppGpp by nudix pyrophosphatase modulates the transition of growth phase in the bacterium Thermus thermophilus.
TREX2 双链 DNA 加工的结构见解
Nucleic Acids Res. 2018 Dec 14;46(22):12166-12176. doi: 10.1093/nar/gky970.
4
Resistance to UV Irradiation Caused by Inactivation of and Genes in Thermus thermophilus.嗜热栖热菌中 和 基因失活导致的抗紫外线辐射。
J Bacteriol. 2018 Jul 25;200(16). doi: 10.1128/JB.00201-18. Print 2018 Aug 15.
5
Structural basis for overhang excision and terminal unwinding of DNA duplexes by TREX1.TREX1 介导的 DNA 双链末端突出切除和末端解旋的结构基础。
PLoS Biol. 2018 May 7;16(5):e2005653. doi: 10.1371/journal.pbio.2005653. eCollection 2018 May.
6
Roles of Mn-catalase and a possible heme peroxidase homologue in protection from oxidative stress in Thermus thermophilus.嗜热栖热菌中锰过氧化氢酶和一种可能的血红素过氧化物酶同系物在抵御氧化应激中的作用。
Extremophiles. 2015 Jul;19(4):775-85. doi: 10.1007/s00792-015-0753-2. Epub 2015 May 22.
7
Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.结构洞察 DNA 修复由 RNase T-一个外切酶处理 3' 端的结构 DNA 在修复途径。
PLoS Biol. 2014 Mar 4;12(3):e1001803. doi: 10.1371/journal.pbio.1001803. eCollection 2014 Mar.
8
In vivo, in vitro, and x-ray crystallographic analyses suggest the involvement of an uncharacterized triose-phosphate isomerase (TIM) barrel protein in protection against oxidative stress.体内、体外和 X 射线晶体学分析表明，一种未被描述的磷酸丙糖异构酶（TIM）桶状蛋白参与了对抗氧化应激的保护。
J Biol Chem. 2011 Dec 2;286(48):41636-41646. doi: 10.1074/jbc.M111.293886. Epub 2011 Oct 7.
9
Inactivation of the DNA repair genes mutS, mutL or the anti-recombination gene mutS2 leads to activation of vitamin B1 biosynthesis genes.失活 DNA 修复基因 mutS、mutL 或抗重组基因 mutS2 会导致维生素 B1 生物合成基因的激活。
PLoS One. 2011 Apr 28;6(4):e19053. doi: 10.1371/journal.pone.0019053.
10
Molecular mechanisms of the whole DNA repair system: a comparison of bacterial and eukaryotic systems.整个DNA修复系统的分子机制：细菌和真核生物系统的比较
J Nucleic Acids. 2010 Oct 14;2010:179594. doi: 10.4061/2010/179594.
Nudix焦磷酸酶对ppGpp的降解调节嗜热栖热菌的生长阶段转变。
J Biol Chem. 2009 Jun 5;284(23):15549-56. doi: 10.1074/jbc.M900582200. Epub 2009 Apr 3.
4
DNA binding induces active site conformational change in the human TREX2 3'-exonuclease.DNA结合会诱导人TREX2 3'-核酸外切酶的活性位点构象发生变化。
Nucleic Acids Res. 2009 Apr;37(7):2411-7. doi: 10.1093/nar/gkp025. Epub 2009 Mar 24.
5
Regulatory RNAs in bacteria.细菌中的调控RNA
Cell. 2009 Feb 20;136(4):615-28. doi: 10.1016/j.cell.2009.01.043.
6
Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair.内切核酸酶V与DNA的结构揭示了脱氨基腺嘌呤修复的起始。
Nat Struct Mol Biol. 2009 Feb;16(2):138-43. doi: 10.1038/nsmb.1538. Epub 2009 Jan 11.
7
Orchestration of Haemophilus influenzae RecJ exonuclease by interaction with single-stranded DNA-binding protein.通过与单链DNA结合蛋白相互作用对流感嗜血杆菌RecJ核酸外切酶进行调控。
J Mol Biol. 2009 Feb 6;385(5):1375-96. doi: 10.1016/j.jmb.2008.11.041. Epub 2008 Nov 27.
8
Cooperative DNA binding and communication across the dimer interface in the TREX2 3' --> 5'-exonuclease.TREX2 3'→5'外切核酸酶中通过二聚体界面的协同DNA结合与通讯。
J Biol Chem. 2008 Aug 1;283(31):21441-52. doi: 10.1074/jbc.M803629200. Epub 2008 Jun 5.
9
An O6-methylguanine-DNA methyltransferase-like protein from Thermus thermophilus interacts with a nucleotide excision repair protein.嗜热栖热菌中的一种O6-甲基鸟嘌呤-DNA甲基转移酶样蛋白与一种核苷酸切除修复蛋白相互作用。
J Biochem. 2008 Aug;144(2):267-77. doi: 10.1093/jb/mvn065. Epub 2008 May 15.
10
Mass spectrometric characterization of proteins modified by nitric oxide-derived species.一氧化氮衍生物质修饰的蛋白质的质谱表征
Methods Enzymol. 2008;440:3-15. doi: 10.1016/S0076-6879(07)00801-4.